Alliinase from Ensifer adhaerens and Its Use for Generation of Fungicidal Activity

AMB Express

Table 3 Substrate specificity of alliinase from E. adhaerens FERM P-19486

Substrate	Kinetic constants
	V _max ^a (units/mg)	K m (mM)	V _max /K m
(±)-Alliin	90.0	2.3	39.1
(+)-Alliin	18.5	5.7	3.2
S-Allyl-L-cysteine	66.1	10.6	6.2
S-Methyl-L-cysteine	15.3	-^b	-
(±)-S-Methyl-L-cysteine sulfoxide	53.6	-	-
S-Ethyl-L-cysteine	29.7	-	-
L-Cysteine	1.6	-	-

^a The V _max value was obtained by the rate of pyruvic acid production in the standard reaction mixture containing each substrate at 40 mM.
^b Not tested.