Substrate
|
Conversion
a
[%]
|
Specific activity
b
[mU/mg]
|
---|
(+)-Camphor ((+)-1)
|
66
|
0.9
|
(-)-Camphor ((-)-1)
|
25
|
0.3
|
Norcamphor (13)
|
98
|
1.3
|
(±)-cis-bicyclo [3.2.0] hept-2-en-6-one (14)
|
100
|
1.4
|
(R,R)-bicyclo [2.2.1] heptane-2,5-dion (15)
|
94
|
0.06
|
-
aConversion was determined by GC-MS analysis of biocatalysis samples with crude extract from E. coli cells overexpressing DKCMO at a substrate concentration of 1 mM after 23 h.
-
bSpecific activity was calculated from conversion of substrate by pure enzyme determined by GC-MS at different points of time.