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Table 2 conversion of several bicyclic ketones by crude cell extract and specific activities for pure 2,5-DKCMO

From: Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007

Substrate

Conversion a [%]

Specific activity b [mU/mg]

(+)-Camphor ((+)-1)

66

0.9

(-)-Camphor ((-)-1)

25

0.3

Norcamphor (13)

98

1.3

(±)-cis-bicyclo [3.2.0] hept-2-en-6-one (14)

100

1.4

(R,R)-bicyclo [2.2.1] heptane-2,5-dion (15)

94

0.06

  1. aConversion was determined by GC-MS analysis of biocatalysis samples with crude extract from E. coli cells overexpressing DKCMO at a substrate concentration of 1 mM after 23 h.
  2. bSpecific activity was calculated from conversion of substrate by pure enzyme determined by GC-MS at different points of time.
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