Figure 3

Vector 2,5-DKCMO_pET-28 for expression of recombinant 2,5-DKCMO from P. putida NCIMB 10007 under control of T7 promoter in E. coli BL21. The 2,5-DKCMO-gene was introduced using the sites of restriction endonucleases Nde I and Xho I for cloning.