Figure 4

Thermal stability for each domain of CYP102A1 variants. Enzymes (2 μM) were incubated at different temperatures between 25 and 70°C for 20 min with subsequent cooling to 4°C in a PCR thermocycler. The stability of the heme domain was calculated from heat-inactivation curves of CO-binding difference spectra. The stability of the reductase domain was calculated from the reduction of ferricyanide catalyzed by reductase activity.