Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax

AMB Express

Table 2 Degradative actions of microbial and commercial xylanolytic activities on A. donax biomass and transglycosylation reactions

	Biocatalyst	S/B	RS%	Xyl%	Glc%	Useful results
1	TL-xylanase	3.3	23.5	2.26	8.6	xylobiose 3.3 g/L
1	TL-xylanase	3.3	23.5	2.26	8.6	pentasaccharide 1 (Figure 2), 2 g/L
2	TM-xylanase	2.6	38	0	0	-
3	CFE-TT	3.5	90	19.2	16.9	-
4	CFE-TT	1.2	100	28.6	6.8	-
5	CFE-TT	3.5	20	8.7	7.8	tetrasaccharide 2 (Figure 2) 2.64 g/L
6	CFE-TN	1.2	75	40.5	15.2	see Figure 3
7	ES-GTM1b	13.5	73.5	26.5	0	see Figure 3
8	ES-GTM1a	36.3	63.1	9.85	0	see Figure 3
			Comment
9	TM-xylanase	7.7	Formation of mono and dixylosylated acceptor
10	CFE-TN	1.2	Formation of mono and dixylosylated acceptor
11	ES-GTM1	13.5	Formation of mono, di and trixylosylated acceptor
12	CFE-TT	3.5	Formation of mono and dixylosylated acceptor

Data are expressed in percentages using means of the values obtained by chemical and enzymatic determinations conducted in triplicate that did not differ by more than 5%. S/B = amount of biocatalyst, mg substrate/mg total protein; RS = Reducing sugars yield % (DNS); TL = Thermomyces lanuginosus xylanase; TM = Thermotoga maritima xylanase; CFE-TT = cell free extract of Thermoanaeorbacterium thermostercoris; CFE-TN = cell free extract of T. neapolitana; ES-GTM1 = extracellular suspension Geobacillus thermantarcticus.