Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax
© Lama et al.; licensee Springer 2014
Received: 6 June 2014
Accepted: 14 June 2014
Published: 9 July 2014
Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extracts of thermophilic microorganisms (Thermotoga neapolitana, Geobacillus thermantarcticus and Thermoanaerobacterium thermostercoris) is studied using as substrate hemicellulose from one of the most interesting biomass crops, the giant reed (Arundo donax L.). This biomass can be cultivated without competition and a huge amount of rhizomes remains in the soil at the end of cropping cycle (10–15 years) representing a further source of useful molecules. Optimization of the procedure for preparation of the hemicellulose fraction from rhizomes of Arundo donax, is studied. Polysaccharidases from crude extracts of thermophilic microorganisms revealed to be suitable for total degradative action and/or production of small useful oligosaccharides from hemicelluloses from A. donax. Xylobiose and interesting tetra- and pentasaccharide are obtained by enzymatic action in different conditions. Convenient amount of raw material was processed per mg of crude enzymes. Raw hemicelluloses and pretreated material show antioxidant activity unlike isolated tetra- and pentasaccharide. The body of results suggest that rhizomes represent a useful raw material for the production of valuable industrial products, thus allowing to increase the economic efficiency of A. donax cultivation.
KeywordsBiorefinery Xylan Thermophilic enzymes Xylanases Xylooligosaccharides Arundo donax
Among interesting thermostable enzymes isolated from microorganisms, polysaccharidases are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. Microorganisms of the genus Thermoanaerobacterium and others of thermophilic or hyperthermophilic origin, are relevant for their abilities to bioconvert carbohydrates. In the framework of a research project focused on bioprospecting for thermophilic microorganisms, a new species, T. thermostercus (designated as Buff), was recently isolated from buffalo-dung and classified as a new member of Thermoanaerobacterium. The optimum growth temperature, pH and NaCl concentration were, respectively, 60°C, 6.5 and 0.5% (w/v). As carbon sources this strain utilized glucose, mannose, xylose, maltose, cellobiose, sucrose and xylan (Romano et al. ). Geobacillus thermantarcticus, a thermophilic bacterium isolated from Antarctic geothermal soil near the crater of Mount Melbourne, produced extracellular xylanase (1,4-β-D-xylan xylanohydrolase; E.C. 22.214.171.124) and β-xylosidase (1,4-β-D-xylan xylohydrolase; E.C. 126.96.36.199); the studied action of these two enzymes on commercial xylan gave only xylose (Lama et al. ). Glycosyl hydrolases in Thermotoga neapolitana resulted interesting in the enzymatic process for the cleavage of xylan to smaller oligosaccharides and also in their coupling to aglycones by transglycosylating activity achieved under certain reaction conditions (Tramice et al. ).
In biomass refinery, lignocellulosic material is fractionated to obtain the main components (cellulose, hemicelluloses, and lignin). Xylan is the major hemicellulosic component. Polysaccharidic chain is composed by a backbone of β-1,4-xylose units in pyranose form, decorated on different position with 4-O-methyl-D-glucuronic acid and L-arabinofuranose units. This skeleton can also be extensively esterified with acetic or ferulic acids; the latter in some species, provides a chemical link between hemicellulose and lignin. D-xylose and L-arabinose as end products are derived by biorefinery enzymatic processes from xylan of lignocellulosic biomasses. However, up until recently biomass-to-fuel investigation has neglected heteroxylan from lignocellulosic. This has been attributed to a number of reasons pointing out that (i) a sub-optimal settings for material extraction or (ii) the slow development of enzymatic processes and (iii) the scarce exploitation of derived products, characterize the field of pentose-specific conversion/utilization technologies. Specific xylanase actions on xylan could lead to important oligosaccharides (Dumon et al. ). Interesting bioactivities have been reported for both poly- and oligosaccharidic fragments. Immunostimulating effects and anti-inflammatory and antimicrobial activities have been reported for arabino(glucurono)xylans isolated from different sources (Christakopoulosa et al. ). A nutraceutical perspective has been also considered for these compounds (Saeed et al. ). A gastroprotective arabinoxylan from sugarcane bagasse was isolated (Mellinger-Silva et al. ). A structure-activity relationship study on the role of 4-O-methyl glucuronic acid (MeGlcUA) in regulating aggregation of β-polyxylosides of (9H-fluoren-9-yl)-methanol obtained via the transglycosylating action of Thermotoga neapolitana β-xylanase, was also studied. An anti-proliferative test of these compounds on human epithelial EFO 27 ovarian cancer cells indicated that the presence of MeGlcUA modulates biological activity, while its absence induced molecular aggregation (Tramice et al. ).
With the increasing demand of ligno-cellulosic materials in processes such as co-generation, production of biofuel and compost from municipal solid wastes, etc., a competition for land used for food production is observed with potential risks for food security in wide areas of the world (van der Horst and Vermeylen ). Among the different biomass crops for ligno-cellulosic materials, one of the most interesting is giant reed (Arundo donax L.), because it is tolerant to a wide range of environmental stresses so that it can be cultivated on marginal, degraded or contaminated lands thus reducing competition with food crops which generally require a better quality arable land (Di Nasso et al. ; Fiorentino et al. ; Diodato et al. ). Arundo donax is suggested in projects (i.e. Ecoremed 2011. LIFE11/ENV/IT/275), (Fagnano ) of phytoremediation of polluted soils (Bonanno et al. ). The most studied part of Arundo donax is represented by shoots (reeds) of interest in bioethanol, biodiesel (Pirozzi et al. ) or biopolymer production (Galletti et al. ). However at the end of cropping cycle of a giant reed stand (10–15 years), a huge amount of rhizomes remains in the soils and they have to be eliminated to afford further agricultural utilizations. These rhizomes could represent a further source of useful molecules for bioenergy or biochemicals (Di Nasso et al. ).
In the present article an overview of degradative actions of different enzymatic extract on hemicelluloses from rhizomes of Arundo donax is presented, comparing the processes acted by mesophilic and thermophilic biocatalysts. Convenient set up of a degradative processes with/without production of small useful oligosaccharides are reported.
Total carbohydrate content was determined by Bernfeld () method using a xylose-based calibration curve. Xylose was determined by D-xylose assay kit (Megazyme), specifically designed to measure this sugar in samples containing up to 5 mg of glucose in the final assay reaction without any interference. Glucose was assayed enzymatically by GOPOD-FORMAT, Megazyme. Protein concentration was determined by the method of Bradford () using bovine serum albumin as the standard. Thermomyces lanuginosus xylanase, (X2753 2500 U/g) was obtained from Sigma. Thermotoga maritima endo-1,4-β-xylanase, 22 U/mg of protein (wheat arabinoxylan at pH 5.0 and 40°C) was obtained from Megazyme.
1H NMR spectra were recorded at 600.13 MHz on a Bruker DRX-600 spectrometer, equipped with a TCI Cryo Probe TM, fitted with a gradient along the Z-axis. Spectra in D2O were referenced to internal sodium 3-(trimethyl-silyl)-(2,2,3,3-2H4) propionate (Aldrich, Milwaukee, WI). 13C NMR, JMOD-1H and COSY, TOCSY, HSQC, HSQC-EDITED, HMBC (3 J: 7 and 10 Hz), NOESY (mixing time at 100, 200, 300 msec) experiments were used for structural determination.
Mass spectra were acquired on a microQ-Tof mass spectrometer coupled with an Alliance HPLC (Waters, Milford, MA) equipped with an ESI source.
Microorganism and culture conditions
Geobacillus thermantarcticus (DSM 9572) isolated from Antarctic geothermal soil was grown in a flask (2 L) at 60°C on a medium containing xylan at pH 6.0 as previously reported (Nicolaus et al. ). Thermoanaerobacterium thermostercoris strain BUFF was grown at 60°C in a 40 L fermentor (B. Brown Biotech International Micro DCU400) as previously reported (Romano et al. ). Thermotoga neapolitana DSM 4359 obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) was grown at 80°C in a 40-L fermentor (B. Brown Biotech International Micro DCU400) as previously reported (Tramice et al. ).
Microbial growth was monitored turbidimetrically at 540 nm and cells were harvested in late exponential growth phase and collected by continuous centrifugation using an Alpha-Laval centrifuge and then pelleted by centrifugation at 10,000 rpm for 30 min and frozen.
Preparation of crude enzymatic extracts
Thermoanaeorbacterium thermostercoris (CFE-TT protein 11.4 mg/ml), and Thermotoga neapolitana cell free extracts (CFE-TN protein 34.4 mg/ml) were prepared in acetate buffer 50 mM pH 5.5 disrupting the cells by usual techniques (freeze-thawing, lysozyme 100 μg/ml). Geobacillus thermantarcticus extracellular suspension (ES-GTM1, protein 0.37 mg/ml) was prepared by adding ammonium sulfate to the cell-free broth (1 L) to 80% saturation. The precipitate was recovered by centrifugation (10,000 g, 1 h, 4°C), dissolved in 50 mM sodium acetate buffer (pH 5.6) and dialyzed overnight against the same buffer.
Arundo donax was grown under Mediterranean climatic conditions: during the growth period (April-November 2012) rainfall was 355 mm and Reference Evapotranspiration was 923 mm and thus water deficit was very severe (568 mm). Rhizomes of A. donax were harvested at the experimental farm of the University “Federico II” Naples and were obtained from a 4 year experimental plantation located in the Sele river plain of Campania Region (Southern Italy, 40°61’N, 14°92’E, 30 m a.s.l.). Rhizome samples were harvested in a sample area of 2 m2, and then chipped, dried at 60°C until constant weight and grinded at 4 mm. Successively, the material was grinded in order to obtain a homogenous fine powder (particle size of about 1 mm) by means of a kitchen mixer (Waring).
Preparation and optimization of hemicellulosic substrates from rhizome of Arundo donax
The hemicellulose fraction was solubilized in alkali medium by continuous magnetic stirring at room temperature; different 2% w/v solutions of rhizome of Arundo donax in potassium hydroxide (KOH, 0.5 N, 1 N and 2 N) were used at different time intervals, 24 h, 48 h, 72 h and 96 h. Experiments for the optimization of extraction procedure were conducted in triplicate; briefly, the grinded dry material was suspended in KOH solutions; afterwards the suspension was sieved (0.5 mm screen) and centrifuged at 10,000 rpm for 40 min at 4°C. The supernatant was treated with same volume of cold ethanol 96% (v/v) added drop wise under stirring. The alcoholic suspension was stored at −20°C overnight and then centrifuged at 10,000 rpm for 1 h at 4°C. The pellet was solubilized in hot distilled water, cooled at room temperature, the pH was adjusted to 7.0 with HCl and dialyzed against distilled water using Spectrapore dialysis tubes (12,000-14,000 MW cut-off), and finally freeze-dried. The lyophilized material (RK72h, RK48h, RK24h) was weighed using a Mettler Toledo analytical balance and the yield was expressed in weight (%, w/w) with respect to the initial dry biomass.
Hydrolysis reactions were conducted using various biocatalysts and hemicellulosic material prepared from A. donax (RK72h) as follows: Thermomyces lanuginosus xylanase, 22 units were dissolved in 3 ml phosphate buffer 50 mM, pH 7 containing 30 mg substrate. Reaction was performed at 34°C under stirring for 26 h and stopped by enzyme denaturation at 100°C (2 min). Thermotoga maritima endo-1,4-β-xylanase, 57 units were dissolved in 1 ml in acetate buffer 50 mM, pH 5.6 containing 10 mg of substrate. Reaction was performed under stirring at 70°C, 24 h and stopped by cooling. Cell free extract (CFE-TT) from Thermoanaeorbacterium thermostercoris (proteins: 11.4 mg/ml), was added to the needed amount of substrate (4 mg/ml of reaction) in acetate buffer 50 mM, pH 5.6. Reaction was performed at 50°C for 24 h under stirring. Cell free extract (CFE-TN) from Thermotoga neapolitana (proteins: 34.4 mg/ml), was added to the needed amount of substrate (4 mg/ml of reaction) in acetate buffer 50 mM, pH 5.6. Reaction was performed at 90°C for 24 h under stirring. Extracellular suspension of Geobacillus thermantarcticus 8 ml, ES-GTM1b, (proteins: 0.37 mg/ml) was reacted with 40 mg of substrate solubilized in 2 ml phosphate buffer 50 mM, pH 6, at 70°C for 24 h. In a different condition, ES-GTM1a, 1.5 ml were reacted with 20 mg of substrate solubilized in 4 ml phosphate buffer 50 mM, pH 6, or at 70°C for 24 h.
Transglycosylation reactions were performed using various biocatalysts as follows: 20 mg of A. donax (RK72h) in 1 ml of appropriate buffer/acetonitrile (87:13 v/v) containing 19.6 mg of 9-fluorene methanol; the reactions started by adding the different biocatalysts (57 U Thermotoga maritima endo-1,4-β-xylanase, at 70°C, 24 h; 500 μL Thermotoga neapolitana cell free extract (CFE-TN) at 70°C, 24 h; 500 μL cell free extract (CFE-TT) from Thermoanaeorbacterium thermostercoris at 50°C, 24 h; 4 ml of extracellular suspension Geobacillus thermantarcticus (ES-GTM1) at 70°C, 24 h) under stirring.
Monitoring of reactions, purification and analysis of products
Hydrolyses reactions were monitored by TLC in solvent A: n-BuOH/AcOH/H2O 6:2:2 v/v, or B: EtOAc/AcOH/2-propanol/HCOOH/H2O, 25:10:5:1:15 by vol, whereas transglycosylation reactions were monitored in solvent C: EtOAc/MeOH/H2O 70:20:10 by vol. Chemical determination of reducing sugars and enzymatic determinations of glucose and xylose were conducted in triplicate. Hydrolysis reaction mixture was concentrated under vacuum and loaded onto a Biogel P-100 column (1 × 35 cm) equilibrated with water and eluted at 0.1 ml/min with water. A second Biogel P-2 column (1 × 47 cm) was performed eluting at 0.3 ml/min with water. The fractions containing the carbohydrates were pooled and freeze-dried. Purified material was subjected to NMR spectroscopy. The reactions of transglycosylation were stopped by cooling and immediately subjected to reverse-phase column chromatography (Merck Lobar RP-18) eluting with water, and then with methanol thus efficiently separating total chromophoric xylosylated fraction and unreacted acceptor in methanol fraction from free saccharides in water. The methanolic fractions were collected and analysed by ESI-MS.
Monosaccharides composition of Arundo donax polysaccharides was determined by GC-MS of acetylated methyl glycosides (Silipo et al. ). Methanolysis was performed in 1.25 M HCl in methanol. In brief, sample (1.5 mg) was solubilized in anhydrous 1.25 M HCl/MeOH (1 ml) and then heated at 80°C overnight. Subsequently, air-dried samples were acetylated with acetic anhydride (50 μl) and pyridine (50 μl) at 100°C for 30 min. Then, samples were dried and washed with methanol in order to evaporate pyridine and dissolved in acetone (400 μl) and 1 μl was used for GC-MS analysis. All GC-MS runs were conducted on a Hewlett-Packard Series II Plus gas chromatograph (column: 30 m × 0.25 mm HP-5MS Hewlett-Packard Co.) coupled with a Hewlett-Packard 5989B mass spectrometer. Electron voltage was set at 70 eV and He was used as gas carrier at a costant flow rate of 1 ml/min. The temperature program used was 150°C for 3 min; from 150 to 280°C at 10°C/min; 280°C for 20 min.
Antimicrobial activity was carried out using liquid culture of E. coli (DSM 498), B. subtilis subsp. spizizenii (DSM 347), and M. luteus (DSM 348). The MIC was determined by a serial dilution, in duplicate, starting from 100 μg/mL to 0.01 μg/mL. Bacterial and yeast growths were observed after 48 h of incubation. Cytotoxic activity was evaluated by the brine shrimp (Artemia salina) test in triplicate with appropriate amounts of samples dissolved in DMSO (1% final volume) to reach final concentrations of 100, 10 and 1 μg/ml, using 10 freshly hatched larvae suspended in 5 ml of artificial seawater (Meyer ). Briefly, for each dose tested, surviving shrimps were left at RT and counted after 24 h, and the data statistically analyzed by the Finney program (Finney ), which affords LD50 values with 95% confidence intervals.
Free-radical scavenging activity was evaluated on pre-treated grinded biomass in DMSO and on RK72h dissolved in H2O at a concentration of 20 mg/mL, and assayed for DPPH test (Blois ). Different amounts (10, 50, 100 and 250 μL) of these solutions were added to 0.7 mL of DPPH in MeOH (6 mg/50 mL; 0.1 mM final concentration) and adjusted to 2 mL final volume with MeOH. The absorbance at 517 nm was determined after 30 min at room temperature and the percent of free radical inhibition was calculated. Fucoidan (Sigma) was used as standard. Free radical scavenging activity of samples was estimated as % inhibition of free radical DPPH. Antioxidant activity of both samples was also determined by DMPD method (Fogliano et al. ). The reaction mixture contained 1 mM DMPD, 0.1 mM ferric chloride in acetate buffer 0.1 M (pH 5.25) in a total volume of 1 mL. The assay temperature was 25°C. The reaction was monitored at 505 nm until absorbance became stable at a value of 0.900 ± 0.100. Samples were dissolved in H2O at a concentration of 20 mg/mL. According to the method, the antioxidant activity of samples was carried out in triplicate on main solution (20 mg/mL) and on its diluted solutions 1:2, 1:5, 1:10. Then, 250 μL of solutions were added to the reaction mixture and the decrease in absorbance, which is proportional to the DMPD+ quenched, was determined after 20 min at room temperature. Fucoidan was used as standard. The antioxidant activity was reported as % inhibition of radical cation DMPD.
Hemicellulosic substrates from rhizomes of Arundo donax: preparation and optimization of the procedure
Aboveground biomass yield of Arundo donax was 39.5 ± 8.1 t/ha and rhizome yield was 40.0 t/ha. With this root:shoot ratio of 1 the results of other researches carried out in Mediterranean conditions on mature giant reed stands (Di Nasso et al. ), were confirmed.
Monosaccharide composition of Arundo donax polysaccharides extracted with KOH 2 N at 72 h
The action of crude homogenates on rhizomes of Arundo donax
Degradative actions of microbial and commercial xylanolytic activities on A. donax biomass and transglycosylation reactions
xylobiose 3.3 g/L
pentasaccharide 1 (Figure 2), 2 g/L
tetrasaccharide 2 (Figure 2) 2.64 g/L
see Figure 3
see Figure 3
see Figure 3
Formation of mono and dixylosylated acceptor
Formation of mono and dixylosylated acceptor
Formation of mono, di and trixylosylated acceptor
Formation of mono and dixylosylated acceptor
1 H and 13 C chemical shifts of compounds in Figure 2
2 Figure 2
In a similar hydrolysis reaction (Table 2, entry 2) conducted using xylanase from T. maritima the production in high amount of xylobiose and small xylooligomers as main products from Arundo biomass, was confirmed. The absence of glucose was due to better purity of enzymatic activity (xylanase).
The reaction using T. thermostercoris was conducted on a preparative scale and stopped at 20% conversion (Table 2, entry 5) for the recovery (2.64 g/L) of an interesting pure tetrasaccharide (2, Figure 2) isolated from the reaction mixture by Biogel P-2 purification. The compound showed a peak in the MS spectrum at m/z 569 [M + Na]+ corresponding to a tetrasaccharide made of pentose sugars. 1H and 13C NMR signals are reported in Table 3. NMR signals of anomeric protons were found in the range 4.35-5.32 ppm and those of anomeric carbon atoms in the range 92.6-108.5 ppm. HMBC spectrum showed long-range correlations between 13C anomeric signal of α-L-Araf and signal of H3 proton of central xylose and the related proton anomeric signal of α-L-Araf and C3 of central xylose (see Table 3 for values). Suitable resolution of spectra for signal assignment of both compounds in Figure 2 was achieved only in dilute conditions (3–4 mg/ml); more concentrated samples furnished unresolved spectra and time-dependent signal shifts. Despite the structural reporter group concept for these structures is widely known (Vliegenthart et al. ), structures in Figure 2 were established by analysis of 1H-13C NMR correlations on dilute samples.
Amount of xylose was formed also in the case of G. thermantarcticus (Table 2, entries 7–8) in accord to the loading of the enzyme. Using high amount of substrate per mg of crude biocatalyst (Table 2, entry 8) interesting oligomers accumulated (from xylobiose to other high molecular oligosaccharides, Figure 3).
Transglycosylation reactions were performed using A. donax RK72h biomass as donor and different biocatalysts (entries 9–12, Table 1). At the end of each reaction, total chromophoric xylosylated fraction was obtained by reverse phase column and analyzed by TLC with authentic standards (Tramice et al. ), and MS spectroscopy. Mono- (m/z 351 [M + Na]+) and di-xylosylated (m/z 483 [M + Na]+) 9-fluorene methanol were identified in the reaction using T. maritima xylanase. The same reaction was performed using T. neapolitana, T. thermostercoris and G. thermantarcticus, crude homogenates. In the latter case mass peak at m/z 615 [M + Na]+ due to trixylosylated 9-fluorene methanol was also observed. Although not optimized for the yield, these reactions for the formation of the xylosylated products starting with Arundo biomass are of interest for enlarging the portfolio of pentose-specific conversion/utilization technologies (Dumon et al. ).
Samples of raw materials, extracts of rhizome and purified compounds from hydrolysis reactions were assayed for their antimicrobial, cytotoxic and antioxidant activities. Antimicrobial activity was evaluated on three bacterial strains, E. coli (DSM 498), B. subtilis subsp. spizizenii (DSM 347), and M. luteus (DSM 348). All samples did not show any significant antimicrobial activity. Brine shrimp assay was utilized as indicative of cytotoxicity (Meyer et al. ). The brine shrimp is a marine crustacean used at stage of larvae (48 h after hatching). LD50 is the minimum lethal dose for which we observe a 50% mortality of larvae. Results obtained by using this test showed that all samples were not toxic on brine shrimp larvae; in fact after 24 h of exposure to samples at concentration of 100, 10 and 1 μg/ml, all larvae were live and motile.
Antioxidant activities of the raw material and of the extract of rhizome RK72h compared to that observed using fucoidan were good both in DMPD and DPPH assays. Inhibition of DMPD radical cation (43 and 35%) and DPPH radical (68 and 45%) were observed for raw material and extract of rhizome RK72h, respectively at the maximum tested amount (4 mg). Dose dependent activity was observed at lower concentrations (2 to 0.1 mg). Unfortunately purified compounds (Figure 2) did not exhibit significant antioxidant activity.
In this article the optimization of hemicellulosic substrate preparation from rhizome of Arundo donax has been reported. Hemicellulosic fraction represented ca. 20% of the dried biomass and occurred in a partially de-branched form after alkaline extraction, as indicated by Ara/Xyl ratio (Verbruggen et al. ) and 13C NMR spectroscopy.
The production of two different interesting oligosaccharides was possible with both commercial (xylobiose, pentasaccharide 1) and thermophilic microorganisms (tetrasaccharide 2) from this hemicellulose fraction. 1H-13C NMR correlations of products were studied for precise structural assignment. Significant amounts of low molecular mass compounds, xylobiose and trace amounts of xylose were liberated from birch wood xylan after 24 h (Lin et al. ) using Thermomyces lanuginosus enzyme; however cellulase activity could contaminate this endo-β-(1 → 4)-xylanase preparation (Subramaniyan and Prema ) explaining the results obtained. Xylanase from Thermotoga maritima is an extremely thermostable enzyme which hydrolyzed xylo-oligosaccharides and xylans to yield predominantly xylobiose as end product (Jiang et al. ). Xylobiose and small xylooligomers as main products in high amount from Arundo biomass confirmed this activity. The absence of glucose can be explained by better purity of enzymatic preparation (xylanase) with respect to T. lanuginosus reaction. As far as T. neapolitana CFE-TN and T. thermostercoris CFE-TT were concerned, presence of xylanases, xylosidases, glucuronidases and arabinofuranosidases were confirmed in the crude extracts by the action on suitable chromophoric and polysaccharidic substrates (data not shown). The results on Arundo indicated also that action of β-glucosidases is dependent on enzymatic load in the reaction mixture, and this phenomenon could be analyzed with respect to the known interrelation of cellulases and xylanases in the bioconversion of lignocellulosic material (Hu et al. ). All these data are of great interest in the view of research for biocatalyst suitable for complete saccharification of the cellulose and hemicellulose components in raw material to fermentable sugars.
The production of the transxylosylated products from Arundo was also possible with thermophilic enzymes and this is of further interest for enlarging pentose-specific conversion/utilization technologies.
In conclusion rhizomes represent a useful raw material for the production of valuable industrial products increasing the economic and energetic efficiency of A. donax cultivation. Antioxidant activities of the raw material and hemicellulose fraction from rhizomes compared to that observed using fucoidan are good both in DMPD and DPPH assays.
The work included in this article is part of the project PON01_01966 “Filiere agro-industriali integrate ad elevata efficienza energetica per la messa a punto di processi di Produzione Eco-compatibili di Energia e Bio-chemicals da fonte rinnovabile e per la valorizzazione del territorio (EnerbioChem)” 28/10/2011 prot. n. 881/Ric - Programma Operativo Nazionale (PON) “Ricerca e Competitività 2007 – 2013” Regioni Convergenza. We thank D. Melck, E. Castelluccio and A. Esposito (NMR service of Istituto di Chimica Biomolecolare, CNR, Pozzuoli, Italy) for their skilful assistance.
- Bernfeld P: Amylases α and β. In Colowick S. Edited by: Kaplan N. Methods in Enzymology Academic Press, New York; 1955.Google Scholar
- Blois MS: Antioxidant determinations by the use of a stable free radical. Nature 1958, 26: 1199–1200. doi:10.1038/1811199a0 10.1038/1811199a0View ArticleGoogle Scholar
- Bonanno G, Cirelli GL, Toscano A, Lo Giudice R, Pavone P: Heavy metal content in ash of energy crops growing in sewage-contaminated natural wetlands: potential applications in agriculture and forestry? Sci Total Environ 2013, 452–453: 349–354. doi:10.1016/j.scitotenv.2013.02.048 10.1016/j.scitotenv.2013.02.048View ArticlePubMedGoogle Scholar
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72: 248–254. doi:http://dx.doi.org/10.1016/0003–2697(76)90527–3 10.1016/0003-2697(76)90527-3View ArticlePubMedGoogle Scholar
- Christakopoulosa P, Katapodisa P, Kalogerisa E, Kekosa D, Macrisa BJ, Stamatisb H, Skaltsac H: Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases. Int J Biol Macromol 2003, 31: 171–175. doi:http://dx.doi.org/10.1016/S0141–8130(02)00079-X 10.1016/S0141-8130(02)00079-XView ArticleGoogle Scholar
- Di Nasso NNO, Roncucci N, Bonari E: Seasonal dynamics of aboveground and belowground biomass and nutrient accumulation and remobilization in giant reed ( Arundo donax L.): a three-year study on marginal land. Bioenergy Res 2013, 6(2):725–736. doi:10.1007/s12155–012–9289–9 10.1007/s12155-012-9289-9View ArticleGoogle Scholar
- Diodato N, Fagnano M, Alberico I: CliFEM – climate forcing and erosion response modelling at long-term sele river research basin (Southern Italy). Nat Hazards Earth Syst Sci 2009, 9: 1693–1702. doi:10.5194/nhess-9–1693–2009 10.5194/nhess-9-1693-2009View ArticleGoogle Scholar
- Dumon C, Song L, Bozonnet S, Fauré R, O’Donohue MJ: Progress and future prospects for pentose-specific biocatalysts in biorefining. Process Biochem 2012, 47(3):346–357. doi:10.1016/j.procbio.2011.06.017 10.1016/j.procbio.2011.06.017View ArticleGoogle Scholar
- Fagnano M: Implementation of eco-compatible protocols for agricultural soil remediation in litorale Domizio-Agro aversano NIPS. 2014.Google Scholar
- Finney DJ: Statistical aspects of monitoring for dangers in drug therapy. Methods Inf Med 1971, 10: 1–8.PubMedGoogle Scholar
- Fiorentino N, Impagliazzo A, Ventorino V, Pepe O, Piccolo A, Fagnano M: Biomass accumulation and heavy metal uptake of giant reed on polluted soil in southern Italy. J Biotechnol 2010, 150: 261. 10.1016/j.jbiotec.2010.09.155View ArticleGoogle Scholar
- Fogliano V, Verde V, Randazzo G, Ritieni A: Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J Agric Food Chem 1999, 47: 1035–1040. doi:10.1021/jf980496s 10.1021/jf980496sView ArticlePubMedGoogle Scholar
- Galletti AMR, Antonetti C, Ribechini E, Colombini MP, Di Nasso NNO, Bonari E: From giant reed to levulinic acid and γ-valerolactone: a high yield catalytic route to valeric biofuels. Appl Energy 2013, 102: 157–162. doi:10.1016/j.apenergy.2012.05.061 10.1016/j.apenergy.2012.05.061View ArticleGoogle Scholar
- Hu J, Arantes V, Saddler JN: The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect? Biotechnol Biofuels 2011, 4: 36–49. doi:10.1186/1754–6834–4-36 10.1186/1754-6834-4-36PubMed CentralView ArticlePubMedGoogle Scholar
- Jiang ZQ, Deng W, Zhu YP, Li LT, Sheng YJ, Hayashi K: The recombinant xylanase B of Thermotoga maritima is highly xylan specific and produces exclusively xylobiose from xylans, a unique character for industrial applications. J Mol Catal B Enz 2004, 27: 207–213. doi:10.1016/j.molcatb.2003.11.012 10.1016/j.molcatb.2003.11.012View ArticleGoogle Scholar
- Lama L, Calandrelli V, Gambacorta A, Nicolaus B: Purification and characterization of thermostable xylanase and β-xylosidase by the thermophilic bacterium Bacillus thermantarcticus. Res Microbiol 2004, 155(4):283–289. doi:10.1016/j.resmic.2004.02.001 10.1016/j.resmic.2004.02.001View ArticlePubMedGoogle Scholar
- Lin J, Ndlovu LM, Singh S, Pillay B: Purification and biochemical characteristics of β-D-xylanase from a thermophilic fungus. Thermomyces lanuginosus-SSBP Biotechnol Appl Biochem 1999, 30: 73–79.PubMedGoogle Scholar
- Mellinger-Silva C, Simas-Tosin FF, Schiavini DN, Werner MF, Baggio CH, Pereira IT, Da Silva LM, Gorin PA, Iacomini M: Isolation of a gastroprotective arabinoxylan from sugarcane bagasse. Bioresour Technol 2011, 102(22):10524–10528. doi:10.1016/j.biortech.2011.08.107 10.1016/j.biortech.2011.08.107View ArticlePubMedGoogle Scholar
- Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL: Brine shrimps: a convenient general bioassay for active plant constituents. Planta Med 1982, 45(5):31–34. doi:10.1055/s-2007–971236 10.1055/s-2007-971236View ArticleGoogle Scholar
- Nicolaus B, Lama L, Esposito E, Manca MC, di Prisco G, Gambacorta A: Bacillus thermantarcticus sp. nov. from mount Melbourne, Antarctica: a novel thermophilic species. Polar Biol 1996, 16: 101–104. doi:10.1007/s003000050034Google Scholar
- Pastell H, Tuomainen P, Virkki L, Tenkanen M: Step-wise enzymatic preparation and structural characterization of singly and doubly substituted arabinoxylo-oligosaccharides with non-reducing end terminal branches. Carbohydr Res 2008, 343(18):3049–3057. doi:http://dx.doi.org/10.1016/j.carres.2008.09.013 10.1016/j.carres.2008.09.013View ArticlePubMedGoogle Scholar
- Peng F, Peng P, Xu F, Sun R-C: Fractional purification and bioconversion of hemicelluloses. Biotechnol Adv 2012, 30: 879–903. doi:10.1016/j.biotechadv.2012.01.018 10.1016/j.biotechadv.2012.01.018View ArticlePubMedGoogle Scholar
- Pirozzi D, Yousuf A, Fagnano M, Mori M, Fiorentino N, Impagliazzo A: Lipids production by yeast grown on giant reed biomass. J Biotechnol 2010, 150S: 167–168. doi:10.1016/j.jbiotec.2010.08.434 10.1016/j.jbiotec.2010.08.434View ArticleGoogle Scholar
- Romano I, Dipasquale L, Orlando P, Lama L, D'Ippolito G, Pascual J, Gambacorta A: Thermoanaerobacterium thermostercus sp. nov., a new anaerobic thermophilic hydrogen-producing bacterium from buffalo-dung. Extremophiles 2010, 14(2):233–240. doi:10.1007/s00792–010–0303-x 10.1007/s00792-010-0303-xView ArticlePubMedGoogle Scholar
- Saeed F, Pasha I, Anjum FM, Sultan MT: Arabinoxylans and arabinogalactans: a comprehensive treatise. Crit Rev Foods Sci Nutr 2011, 51(5):467–476. doi:10.1080/10408391003681418 10.1080/10408391003681418View ArticleGoogle Scholar
- Silipo A, Sturiale L, De Castro C, Lanzetta R, Parrilli M, Garozzo D, Molinaro A: Structure of the lipo-polysaccharide isolated from the novel species Uruburuella suis . Carbohydr Res 2012, 357: 75–82. doi:10.1016/j.carres.2012.05.010 10.1016/j.carres.2012.05.010View ArticlePubMedGoogle Scholar
- Subramaniyan S, Prema P: Biotechnology of microbial xylanases: enzymology, molecular biology and application. Crit Rev Biotechnol 2002, 22: 33–46. 10.1080/07388550290789450View ArticlePubMedGoogle Scholar
- Tramice A, Melck D, Virno A, Randazzo A, Motta A, Trincone A: Enzymatic synthesis and 3-D structure of anti-proliferative acidic (MeGlcUA) xylotetrasaccharide. J Mol Catal B Enzym 2009, 61(3–4):129–135. doi:10.1016/j.molcatb.2009.05.005 10.1016/j.molcatb.2009.05.005View ArticleGoogle Scholar
- Tramice A, Pagnotta E, Romano I, Gambacorta A, Trincone A: Transglycosylation reactions using glycosyl hydrolases from thermotoga neapolitana, a marine hydrogen-producing bacterium. J Mol Catal B Enz 2007, 47(1–2):21–27. doi:10.1016/j.molcatb.2007.03.005 10.1016/j.molcatb.2007.03.005View ArticleGoogle Scholar
- van der Horst D, Vermeylen S: Spatial scale and social impacts of biofuel production. Biomass Bioenerg 2011, 35(6):2435–2443. doi:10.1016/j.biombioe.2010.11.029 10.1016/j.biombioe.2010.11.029View ArticleGoogle Scholar
- Verbruggen MA, Beldman G, Voragen AGJ: The selective extraction of glucuronoarabinoxylans from Sorghum endosperm cell walls using barium and potassium hydroxide solutions. J Cereal Sci 1995, 21: 271–282. doi:10.1006/jcrs.1995.0030 10.1006/jcrs.1995.0030View ArticleGoogle Scholar
- Vliegenthart JFG, Hoffmann RA, Kamerling JP: A 1 H NMR spectroscopic study on oligosaccharides obtained from arabinoxylans. In Xylans and Xylanases Proceedings of an International Symposium Wageningen, December 1991. Edited by: Vissert J. Elsevier Science Publisher, The Netherlands; 1992:17.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.