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  1. Original article

    Mannan endo-1,4-β-mannosidase from Kitasatospora sp. isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

    Mannan endo-1,4-β-mannosidase (commonly known as β-mannanase) catalyzes a random cleavage of the β-d-1,4-mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocel...

    Nanik Rahmani, Norimasa Kashiwagi, JaeMin Lee, Satoko Niimi-Nakamura, Hana Matsumoto, Prihardi Kahar, Puspita Lisdiyanti, Yopi, Bambang Prasetya, Chiaki Ogino and Akihiko Kondo

    AMB Express 2017 7:100

    Published on: 19 May 2017

  2. Original article

    Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460

    A bioactive strain Streptomyces diastatochromogenes KX852460 was selected for the production of secondary metabolites to control the target spot disease on tobacco leaves, caused by the Rhizoctonia solani AG-3. P...

    Taswar Ahsan, Jianguang Chen, Yuanhua Wu and Muhammad Irfan

    AMB Express 2017 7:96

    Published on: 15 May 2017

  3. Original article

    Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

    To improve the temperature characteristics and catalytic efficiency of a glycoside hydrolase family (GHF) 11 xylanase from Aspergillus oryzae (AoXyn11A), its variants were predicted based on in silico design. Fir...

    Xue-Qing Li, Qin Wu, Die Hu, Rui Wang, Yan Liu, Min-Chen Wu and Jian-Fang Li

    AMB Express 2017 7:97

    Published on: 15 May 2017

  4. Original article

    Autolysis of Pichia pastoris induced by cold

    The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most us...

    Yaneth Bartolo-Aguilar, Luc Dendooven, Cipriano Chávez-Cabrera, Luis B. Flores-Cotera, María E. Hidalgo-Lara, Lourdes Villa-Tanaca and Rodolfo Marsch

    AMB Express 2017 7:95

    Published on: 12 May 2017

  5. Original article

    Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica

    Lysine acetylation of proteins, a major post-translational modification, plays a critical regulatory role in almost every aspects in both eukaryotes and prokaryotes. Yarrowia lipolytica, an oleaginous yeast, is c...

    Guangyuan Wang, Lizhong Guo, Wenxing Liang, Zhenming Chi and Lin Liu

    AMB Express 2017 7:94

    Published on: 12 May 2017

  6. Original article

    Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis

    Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies con...

    Justyna Mozejko-Ciesielska, Dorota Dabrowska, Agnieszka Szalewska-Palasz and Slawomir Ciesielski

    AMB Express 2017 7:92

    Published on: 12 May 2017

  7. Original article

    Role of nitrite in the competition between denitrification and DNRA in a chemostat enrichment culture

    Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine t...

    Eveline M. van den Berg, Julius L. Rombouts, J. Gijs Kuenen, Robbert Kleerebezem and Mark C. M. van Loosdrecht

    AMB Express 2017 7:91

    Published on: 11 May 2017

  8. Original article

    Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

    The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was ...

    Waseem Safdar, Muhammad Shamoon, Xinyi Zan, Junaid Haider, Hafiz Rizwan Sharif, Muhammad Shoaib and Yuanda Song

    AMB Express 2017 7:85

    Published on: 20 April 2017

  9. Original article

    Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress

    Stress, including both psychological and physical stimulation, can cause changes in the microbiota and mucosal function of the gastrointestinal system. There are few research studies available about the faecal...

    Shiyin Li, Zelin Wang, Yun Yang, Sha Yang, Chenchen Yao, Kaiyun Liu, Sixin Cui, Quanming Zou, Hongwu Sun and Gang Guo

    AMB Express 2017 7:82

    Published on: 17 April 2017

  10. Original article

    Degradation and inactivation of Shiga toxins by nitrogen gas plasma

    Shiga toxin (Stx)-producing Escherichia coli (STEC) leads to food poisoning by causing hemorrhagic colitis and hemolytic uremic syndrome. Some STEC produce Shiga toxin 1 (Stx1) and/or Shiga toxin 2 (Stx2), a rela...

    Akikazu Sakudo and Yuichiro Imanishi

    AMB Express 2017 7:77

    Published on: 7 April 2017

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