Fig. 2
From: Saccharomyces cerevisiae cellular engineering for the production of FAME biodiesel
The improvement of SAM production. (A): Precise integration site of SAM2 expression cassette. PCR identifications for the integration of SAM2 expression cassette (B) and the deletion of ADO1gene (C), respectively. The integrated mutant yielded a 2712-bp fragment, while the YPH499 yielded a 1135-bp fragment. The ΔADO1 mutant yielded a 1000-bp fragment, while the YPH499 yielded a 2023-bp fragment. D: SAM titres obtained with different engineered strains. E: Curves of cell growth and SAM productions of the mutant strain SC05 and the strain SC03 in shake flask culture