Fig. 1

The improvement of fatty acids production. PCR identifications for the deletion of FAA1(A), FFA4(B) and POX1(C) genes, respectively. The ΔFAA1 mutant yielded a 1000-bp fragment, while the YPH499 yielded a 3103-bp fragment. The ΔFAA4 mutant yielded a 1000-bp fragment, while the YPH499 yielded a 3085-bp fragment. The ΔPOX1 mutant yielded a 1000-bp fragment, while the YPH499 yielded a 3247-bp fragment. D: FFA titres obtained with different engineered strains. Data are mean ± standard deviation (error bars) of three assays. E: Curves of cell growth and FFA productions of the mutant strain SC03 and the wild-type strain in shake flask culture