Table 1 Strains and plasmids used in this study

Strains

S. peucetius ATCC 27,952

Doxorubicin-producing strain

ATCC

S. peucetius SIPI-14

Doxorubicin-producing mutant strain derived from the S. peucetius ATCC 27,952

(Wang et al. 2018)

S. peucetius SIPI-7-14

Doxorubicin-producing mutant strain derived from the S. peucetius SIPI-14

This study

△U1

Knockout gene dnrU in S. peucetius SIPI-7-14

This study

△H4

Knockout gene dnrH in S. peucetius SIPI-7-14

This study

△X1

Knockout gene dnrX in S. peucetius SIPI-7-14

This study

△U1/drrC

Overexpressed gene drrC in △U1

This study

△U1/drrAB

Overexpressed gene drrAB in △U1

This study

△U1/drrD

Overexpressed gene drrD in △U1

This study

△U1/drrABC

Overexpressed gene drrAB and gene drrC in △U1

This study

DH5α

F–Φ80lacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rk-mk+) phoA

supE44 λ-thi-1 gyrA96 relA1

CWBIO, China

S17-1

RP4-2 (Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510

WEIDI, China

Plasmids

Relevant characteristic

Source

pSET152

E. coli–Streptomyces shuttle vector containing oriT, ΦC31 integration site (attP), aac (3) IV and PermE*, Apr^r

(Bierman et al. 1992)

pSET

pSET152 plasmid with deletion of φC31 integration site, used for gene knockout, Apr^r

(Li et al. 2022)

pSET-△dnrU

pSET with two homologous arms flanking from dnrU

This study

pSET-△dnrH

pSET with two homologous arms flanking from dnrH

This study

pSET-△dnrX

pSET with two homologous arms flanking from dnrX

This study

pSET152-drrC

pSET152 with drrC inserted NdeI, under the control of the constitutive promoter PermE*

This study

pSET152-drrAB

pSET152 with drrAB inserted NdeI, under the control of the constitutive promoter PermE*

This study

pSET152-drrABC

pSET152-drrC with drrAB inserted SpeI under the control of the constitutive promoter PermE*

This study

pSET152-drrD

pSET152 with drrD inserted NdeI, under the control of the constitutive promoter PermE*

This study