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Table 2 Effects of high concentrate diet supplemented with different YC levels on in vitro degradability of DM and ruminal fermentation profile after 48 h of incubation

From: Effects of yeast culture on in vitro ruminal fermentation and microbial community of high concentrate diet in sheep

Items

Treatments

SEM

P-value

CON

YC1

YC2

YC3

YC4

Treatment

Linear

Quadratic

IVDMD (%)

69.42

69.64

70.83

72.31

73.46

2.043

0.773

0.309

0.830

pH

5.54b

5.94ab

6.25a

6.03ab

6.14a

0.086

0.022

0.041

0.015

NH3-N (mg/dL)

33.71a

31.84ab

28.67b

27.54b

34.78a

0.879

0.035

0.039

0.021

MCP (mg/mL)

1.87c

2.69a

2.54a

2.04bc

2.21b

0.008

0.005

0.026

0.010

Lactate (mmol/L)

1.56a

1.23ab

0.88b

0.85b

0.92b

0.052

0.017

0.022

0.011

Total VFA (mmol/L)

116.43

126.54

119.43

120.86

119.68

4.569

0.011

0.009

0.027

Acetate (mmol/L)

72.95

76.43

67.84

67.94

69.75

2.837

0.031

0.039

0.014

Propionate (mmol/L)

21.77b

28.37ab

29.53a

30.29a

28.12ab

0.763

0.043

0.078

0.038

Butyrate (mmol/L)

13.28b

14.05ab

15.56a

15.83a

14.65ab

0.401

0.026

0.061

0.015

Isovalerate (mmol/L)

4.88

4.43

3.79

4.11

4.32

0.340

0.266

0.548

0.380

Valerate (mmol/L)

3.55

3.26

2.71

2.69

2.84

0.123

0.304

0.485

0.255

AP

3.35a

2.69b

2.30c

2.24c

2.48b

0.003

0.026

0.039

0.018

NGR

4.07a

3.41b

3.06c

3.10c

3.29b

0.002

0.029

0.040

0.007

  1. DM, dry matter; YC, yeast culture; IVDMD, in vitro dry matter digestibility; NH3-N, ammonia nitrogen; MCP, microbial protein; VFA, volatile fatty acid; SEM, standard error of the mean. CON, fermentation substrate with no YC; YC1, fermentation substrate supplemented with 0.50% YC; YC2, 1%; YC3, 1.5%; YC4, 2%. AP = acetate / propionate. NGR = (acetate + 2 butyrate + valerate) / (propionate + valerate). In the same row, values with different small letters differ significantly (P < 0.05)