Fig. 1From: Use of an on/off tetracycline riboswitch to control protein production in Komagataella phaffiiMap of the plasmids containing the tetracycline aptamer (tc) controlling protein production. A pPICH-lacZ-3tc episomal vector containing lacZ under control of the tc aptamer. B pPICH-ku70-3tc episomal vector containing ku70 under control of the tc aptamer. All sequences were amplified by PCR and cloned into pPICH vector by homologous recombination or via PCRBack to article page