Fig. 5
From: Simeprevir restores the anti-Staphylococcus activity of polymyxins
Mechanism of action underline the synergistical combination between SIM and PB(S)/PE. (A) Membrane permeabilization determination by SYTOX Green uptake. The S. aureus ATCC 43,300 was treated with SIM alone or in combination with PB(S)/PE, 10 µg/mL melittin and DMSO were used as positive and solvent control, respectively. (B) Membrane potential determination by DiSC3(5) staining. (C) Intracellular ROS detection by DCFH-DA probe. (D) Schematic diagram of the possible mechanism by which SIM enhanced the antimicrobial effect of PB(S)/PE. *: P < 0.05; ***: P < 0.001; ****: P < 0.0001