Fig. 4
From: Simeprevir restores the anti-Staphylococcus activity of polymyxins
SIM combined with PB(S)/PE against S. aureus high resistant phenotypes. (A) Phagocytosis of neutrophils against ATCC 43,300. Blue arrows indicate intracellular S. aureus cells. Scale: 20 μm. (B) Intracellular killing effects of SIM combined with PB(S)/PE against ATCC 43,300. (C) Persister cells killing activity by SIM and PB(S)/PE combination. (D) Biofilm formation of USA300 and ATCC 43,300 after treated with SIM alone or in combination with PB(S) detected by crystal violet staining. (E) Quantification of biofilm formation by crystal violet staining. The asterisks indicate the optimal synergistical biofilm inhibitory concentrations of the tested drugs. (F) Biofilm observation by SYTO9/PI staining after treated with SIM alone or in combination with PB(S)/PE [2 µg/mL SIM and 8 µg/mL PB(S) for ATCC 43,300; 1 µg/mL SIM and 16 µg/mL PB(S)] for USA300. Scale: 200 μm. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001