Fig. 3
From: Simeprevir restores the anti-Staphylococcus activity of polymyxins
In vitro toxicity assessments of SIM combined with PB(S) or PE. (A) Human RBC hemolysis determination after 1 h treatment. (B) Representative images of human RBC hemolysis from different groups. (C) CCK-8 assay of 293T cells for different drugs treatment [including 32 µg/mL PE, 32 µg/mL PB(S), and 4 µg/mL SIM]; (D) Cell apoptosis of 293T cells detected by Annexin V-FITC/PI staining. (E, F) Statistical analysis of the apoptotic cell in different groups for Q2 and Q2 + Q4, respectively. (G) Representative images of the apoptotic cell observation by Calcein-AM/PI staining. ns: no statistical significance. ****: P < 0.0001