Table 2 List of functionally annotated proteins from ORFs in the genome of phage vB_EfKS5
From: Potential application of phage vB_EfKS5 to control Enterococcus faecalis and its biofilm in food
ORF | Predicted function | Position (5’-3’) | Amino acid sequence identity/similarity to best homologs | GenBank Accession no. |
|---|---|---|---|---|
ORF 22 | Phage DNA binding protein | 6512–6817 | Enterococcus phage IMEEF1: Phage DNA binding protein | YP_009603915.1 |
ORF 31 | Tail-length tape-measure protein | 8968–9552 | Enterococcus phage vB_EfaH_EF1TV: putative cytidine deaminase/tail length tape-measure protein | AZV00019.1 |
ORF 34 | ATP-dependent metalloprotease | 10,454 − 11,149 | Enterococcus phage SAP6: ATP-dependent metalloprotease | YP_009604001.1 |
ORF 38 | DNA polymerase I | 12,246 − 12,914 | Enterococcus phage IMEEF1: DNA polymerase | YP_009603915.1 |
ORF 40 | HNH homing endonuclease | 13,136 − 13,648 | Enterococcus phage EFap05-1: HNH homing endonuclease | UIE13835.1 |
ORF 42 | DNA polymerase I | 13,747 − 15,345 | Streptococcus phage SP-QS1]:DNA polymerase I | YP_008320536.1 |
ORF 52 | LPS glycosyltransferase | 17,341 − 17,904 | Enterococcus phage EfsWh-1: LPS glycosyltransferase | QAY01541.1 |
ORF 53 | Putative sigma factor | 17,966 − 18,628 | Enterococcus phage vB_EfaS_TV16: putative sigma factor | QIG60321.1 |
ORF 54 | Putative adenylate kinase | 18,621 − 19,190 | Enterococcus phage phiM1EF2: adenylate kinase | BCF74614.1 |
ORF 55 | Crossover junction endodeoxyribonuclease RuvC | 19,187 − 19,765 | Enterococcus phage phiM1EF: putative crossover junction endodeoxyribonuclease RuvC | BCF74615.1 |
ORF 57 | Exonuclease | 20,087 − 21,115 | Enterococcus phage: Entf1: exonuclease | QDB70560.1 |
ORF 58 | HNH endonuclease | 21,108 − 21,548 | Enterococcus phage phiM1EF2: HNH homing endonuclease | BCF74618.1 |
ORF 61 | DNA methylase | 22,083 − 22,835 | Enterococcus phage EFap05-1: DNA methylase | UIE13815.1 |
ORF 62 | DNA helicase | 22,848 − 24,212 | Streptococcus phage SP-QS1: replicative DNA helicase | YP_008320518.1 |
ORF 63 | DNA replication protein | 22,224 − 25,000 | Enterococcus phage Entf1: DNA replication protein | QDB70565.1 |
ORF 64 | Transcriptional regulator | 25,094 − 25,402 | Enterococcus phage IMEEF1: transcriptional regulator | YP_009603955.1 |
ORF 65 | DNA primase | 25,477 − 26,421 | Enterococcus phage SAP6: DNA primase | YP_009604014.1 |
ORF 87 |  N-acetylmuramoyl-L-alanine amidase | 32,053 − 32,766 | Enterococcus phage vB_EfaS_HEf13: N-acetylmuramoyl-L-alanine amidase | AYH92708.1 |
ORF 89 | Tail spike protein | 33,114 − 36,140 | Enterococcus phage EF653P1: tail spike protein | WAX15251.1 |
ORF 90 | Tail fiber protein | 36,153 − 40,145 | Enterococcus phage vB_EfaS_HEf13: tail fiber protein | AYH92711.1 |
ORF 91 | Tail-length tape-measure protein | 40,159 − 43,044 | Enterococcus phage vB_EfaS_HEf13: tail length tape-measure protein | AYH92712.1 |
ORF 95 | Major tail protein | 43,876 − 44,565 | Enterococcus phage BC611: major tail protein | YP_006488747.1 |
ORF 96 | Tail terminator | 44,586 − 45,020 | Streptococcus phage SP-QS1: tail terminator | YP_008320490.1 |
ORF 99 | Head-tail connector family protein | 45,791 − 46,195 | Enterococcus phage Entf1: head-tail connector family protein | QDB70499.1 |
ORF 100 | Major tail protein | 46,255 − 46,695 | Enterococcus phage vB_EfaS_HEf13: major tail protein | AYH92720.1 |
ORF 101 | Major capsid protein | 46,850 − 47,656 | Enterococcus phage vB_EfaS_TV16: major capsid protein | QIG60313.1 |
ORF 102 | Head scaffolding protein | 47,705 − 48,373 | Enterococcus phage vB_EfaS_IME198: head scaffolding protein | YP_009218884.1 |
ORF 104 | Head morphogenesis protein | 48,484 − 49,239 | Enterococcus phage vB_EfaS_TV16: head morphogenesis protein | QIG60312.1 |
ORF 105 | Phage portal protein | 49,251 − 50,786 | Enterococcus phage vB_EfaS_HEf13: Phage portal protein | AYH92724.1 |
ORF 106 | Phage terminase large subunit | 50,843 − 52,114 | Enterococcus phage vB_EfaS_Ef7.1: Phage terminase large subunit | QBZ69408.1 |
ORF 107 | Holin | 52,177 − 52,425 | Enterococcus phage EF-P29: holin | APU00267.1 |
ORF 109 | Phage terminase small subunit | 52,802 − 53,401 | Enterococcus phage vB_OCPT_CCS2: terminase small subunit | UQT01010.1 |
ORF 114 | Methyltransferase | 54,554 − 55,021 | Enterococcus phage SAP6: methyltransferase | YP_009603990.1 |
ORF 116 | putative glutaredoxin | 55,187 − 55,447 | Enterococcus phage EfsWh-1: putative glutaredoxin | QAY01492.1 |