Enhancing a Sphaerobacter thermophilus ω-transaminase for kinetic resolution of β- and γ-amino acids

AMB Express

Table 2 Temperature stability is the temperature which does not inactivate the enzyme after 60 min of incubation, or 24 h, respectively

Variant	pH optimum	Temperature optimum (°C)	Temperature stability 1 h (°C)	Temperature stability 24 h (°C)
WT	8.0	60	60	40
T38V	7.0	60	50	50
S17P	7.0	40	50	40
N70E	8.0	40	60	40
Y47T	8.0	48	50	40
S17P\|T38V	8.0	40	50	40
T38V\|Y47T	8.0	46	50	40
S17P\|T38V\|Y47T	8.0	40	50	40

Only modifications which show a higher activity with at least one substrate compared to the wild type (WT) are listed here