Enhancing a Sphaerobacter thermophilus ω-transaminase for kinetic resolution of β- and γ-amino acids

AMB Express

Table 1 Comparison of the activities of different StoTA variants

Variant	β-HF (U mg⁻¹)	β-HA (U mg⁻¹)	4-AP (U mg⁻¹)	β-L (U mg⁻¹)	β-F (U mg⁻¹)
WT	0.600 ± 0.029	0.300 ± 0.010	0.125 ± 0.015	0.130 ± 0.020	0.186 ± 0.060
T38V	0.610 ± 0.053	0.251 ± 0.015	0.106 ± 0.004	0.191 ± 0.000	0.251 ± 0.017
S17P	0.591 ± 0.035	0.388 ± 0.017	0.176 ± 0.008	0.093 ± 0.003	0.234 ± 0.003
N70E	0.802 ± 0.018	0.317 ± 0.005	0.114 ± 0.003	0.379 ± 0.001	0.362 ± 0.007
Y47T	3.007 ± 0.017	1.981 ± 0.043	0.871 ± 0.168	0.736 ± 0.125	1.141 ± 0.169
S17P\|T38V	3.211 ± 0.009	2.226 ± 0.208	1.146 ± 0.037	1.056 ± 0.008	1.390 ± 0.021
T38V\|Y47T	2.305 ± 0.026	1.591 ± 0.112	0.737 ± 0.014	1.369 ± 0.080	1.227 ± 0.025
S17P\|T38V\|Y47T	0.869 ± 0.016	0.429 ± 0.012	0.127 ± 0.003	0.386 ± 0.025	0.342 ± 0.033

Only modifications showing a higher activity with at least one substrate compared to the wild type (WT) are mentioned here. The specific activities with different amino donors are given with SD, N = 3
β-HF β-homo-phenylalanine, β-HA β-homo-alanine, 4-AP 4-aminopentanoic acid, β-L β-leucine, β-F β-phenylalanine