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Fig. 6 | AMB Express

Fig. 6

From: Developing a vaccine against velogenic sub-genotype seven of Newcastle disease virus based on virus-like particles

Fig. 6

Gating strategy for the flow cytometric analysis of mice T-cells, platelets, and their subpopulations. A single-cell suspension was stained with fluorochrome-conjugated antibodies against CD4-FL4- APC and CD8-FL1-488. Data were collected with a MACSQuant flow cytometer and analyzed with FlowJo Software. Lymphocytes were identified by their scatter properties (FSC-A × SSC-A plot) and doublets were excluded by gating on FSC-A × FSC-H. CD4 + or CD8 + cells represent T-cell subsets in immunized mice with (a) only NDV-VLPs and (b) NDV-VLPs boosted with B1 NDV live vaccine

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