Skip to main content
AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Developing a vaccine against velogenic sub-genotype seven of Newcastle disease virus based on virus-like particles

Fig. 1

A) Enzyme digested products of shuttle vector pFastBac Dual (5238 bp) before cloning on 1% agarose gel. DNA ‎ladder 200 bp-10 kb (200, 400, 600, 800, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10,000; Thermo Science fisher, ‎Germany).‎ B) PCR products of four structural genes of NDV 2018‎‏/‏‎(VIId) Ck/IR/MAM strain on 1% agarose gel; M gene (1094 ‎bp), F gene (1661 bp), and HN gene (1716 bp), respectively, DNA ladder 200 bp-10 kb. C) Enzyme digested products of pFast (5238 bp) + GFP (717 bp) + P2A (54 bp) + F gene (1661 bp) + M gene (1094 bp) = 8764 ‎bp for cloning verification on 1% agarose gel; DNA ladder 200 bp-10 kb (Thermo Sciencefisher, Germany)

Back to article page