Table 1 List of used primers used in this study
Target | Genes | Primers sequences | Product size (bp) | PCR thermal profile (35 cycles) | References | ||
|---|---|---|---|---|---|---|---|
Denaturation | Annealing | Extension | |||||
Virulence genes | invA | F:TCATCGCACCGTCAAAGGAACC R:GTGAAATTATCGCCACGTTCGGGCAA | 284 | 95 °C 30 s | 55 °C 30 s | 72 °C 90 s | Oliveira et al. (2003) |
hilA | F:CGGAAGCTTATTTGCGCCATGCTGAGGTAG R:GCATGGATCCCCGCCGGCAGAGTTGTG | 854 | 95 °C 30 s | 55 °C 30 s | 72 °C 90 s | Cardona-Castro et al. (2002) | |
pefA | F: TGTTTCCGGGCTTGTGCT R: CAGGGCATTTGCTGATTCTTCC | 700 | 95 °C 30 s | 55 °C 30 s | 72 °C 90 s | Murugkar et al. (2003) | |
stn | F:TTGTGTCGCTATCACTGGCAACC R: ATTCGTAACCCGCTCTCGTCC | 617 | 95 °C 30 s | 55 °C 30 s | 72 °C 90 s | ||
Resistance genes | tetA | F:GGTTCACTCGAACGACGTCA R:CTGTCCGACAAGTTGCATGA | 576 | 94 °C 30 s | 55 °C 40 s | 72 °C 45 s | Randall et al. (2004) |
sul1 | F:CGGCGTGGGCTACCTGAACG R:GCCGATCGCGTGAAGTTCCG | 433 | 94 °C 30 s | 54 °C 40 s | 72 °C 45 s | Ibekwe et al. (2011) | |
aadA1 | F: TATCAGAGGTAGTTGGCGTCAT R:GTTCCATAGCGTTAAGGTTTCATT | 484 | 94 °C 30 s | 50–54 °C 40 s | 72 °C 45 s | Randall et al. (2004) | |
ereA | F: GCCGGTGCTCATGAACTTGAG R: CGACTCTATTCGATCAGAGGC | 419 | 94 °C 30 s | 58 °C 30 s | 72 °C 60 s | Van et al. (2008) | |
blaCTX-M | F:ATG TGC AGY ACC AGT AAR GTK ATG GC R:TGG GTR AAR TAR GTS ACC AGA AYC AGC GG | 593 | 94 °C 30 s | 54 °C 40 s | 72 °C 45 s | Archambault et al. (2006) | |
blaTEM | F:ATCAGCAATAAACCAGC R:CCCCGAAGAACGTTTTC | 516 | 94 °C 30 s | 54 °C 40 s | 72 °C 45 s | Colom et al. (2003) | |
blaNDM | F:GGCGGAATGGCTCATCACGA R:CGCAACACAGCCTGACTTTC | 287 | 94 °C 30 s | 55 °C 40 s | 72˚C 30 s | Xia et al. (2012) | |