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Table 1 The amount of purified protein (µg/ml) based on the intensity of fluorescent signals

From: Synthesis of Ni2+-functionalized polydopamine magnetic beads for facilitated purification of histidine-tagged proteins

PDA/Fe3+/Ni2+

Elution 1

PDA/Fe3+/Ni2+

Elution 2

PDA/Fe3+/Ni2+

Elution3

Commerical Columns

Ni-NTA

16,222 ± 105(RFU)

26,170 ± 100(RFU)

4166 ± 2.86(RFU)

20,535 ± 270(RFU)

84.94 (µg/ml)

138.83 (µg/ml)

 

108.28 (µg/ml)