Fig. 6
Purification analysis of Hsp40 protein by magnetic beads and commercial column (Ni-NTA). 12% SDS-PAGE analysis of Hsp40 expression (a) [Lane 1: The protein marker, Lanes 2, 3, and 4: Cell lysate, cell lysate after treatment by PDA, and PDA/Fe3+ nanoparticles (as negative control), respectively], (b) [ Lane 1: The protein marker, Lane 2: eluted protein by commercial column(Ni-NTA), Lane 3: eluted protein by PDA/Fe3+/Ni2+ nanoparticles in run 6, Lane 4, 5, and 6: eluted protein by PDA/Fe3+/Ni2+nanoparticles in run 1, run 2, run 4, and run 5 (reusability), respectively]