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AMB Express
Fig. 5 | AMB Express

Fig. 5

From: Synthesis of Ni2+-functionalized polydopamine magnetic beads for facilitated purification of histidine-tagged proteins

Fig. 5

Purification analysis of eGFP protein using magnetic beads. (a) 12% SDS-PAGE analysis of eGFP expression [Lane 1: before induction, Lanes 2: 16 h after induction in the 5ml culture, lane 3: Protein marker], (b) 15% SDS-PAGE analysis of purified eGFP [Lane1: protein marker, Lane 2: purified eGFP by PDA magnetic beads in elution 2], (c) Western blot analysis of purified GFP protein [Lane 1: marker protein, lane 2 and 3: purified eGFP protein with PDA magnetic beads in elution 1 and 2, respectively, Lane 4: purified eGFP protein with a commercial Ni-NTA column]. The protein marker molecular weights are 180, 135, 100, 75, 63, 48, 35, 25, 17, and 11 kDa

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