Table 1 Purification of 1, 2 catechol dioxygenase from expressed E.coli and P. putida
Step | Volume (mL) | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yeild | Purification Factor | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
E. coli | P. putida | E. coli | P. putida | E. coli | P. putida | E. coli (%) | P. putida (%) | E. coli | P. putida | ||
Crude preparation | 50 | 60.3 | 32.4 | 631.4 | 243.6 | 10.4 | 7.5 | 100 | 100 | – | – |
(NH4)2SO4 | 5 | 13.3 | 9.5 | 547.5 | 195.4 | 41.16 | 20.5 | 86.7 | 80.2 | 3.9 | 2.7 |
DEAE Sephadex | 5 | 4.5 | 4.3 | 336.9 | 130.7 | 74.8 | 30.3 | 61.5 | 66.8 | 1.8 | 1.4 |