Fig. 5From: Biodegradation of isoproturon by Escherichia coli expressing a Pseudomonas putida catechol 1,2-dioxygenase geneThe 12% SDS-PAGE profile under denaturation conditions protein fractions obtained from anion exchange/size-exclusion chromatography of crude and recombinant catechol 1,2 dioxygenase from P. putida and expressed E. coli respectively. Lane M, Blue Starprestained protein marker, lanes 1 and 2 were crude protein from P. putida and expressed E. coli, lane 3 and 4 the purified enzyme from P. putida and expressed E. coliBack to article page