A microbial flora with superior pollutant removal efficiency and its fermentation process optimization

AMB Express

Table 1 Feeding strategy

Batches	Content					Feed time (h)	Feed duration (h)
Batches	Soybean meal (g)	(NH₄)₂SO₄ (g)	CH₃COONa 3H₂O (g)	Vickers salt solution (ml)	Microelement (ml)	Feed time (h)	Feed duration (h)
1	1.75	2.25	37.5	125	25	15	3
2	1.75	2.25	37.5	125	25	15	5
3	0	2.25	37.5	125	25	15	3
4	0	1.125	37.5	125	25	15	3
5	0	6.75	37.5	125	25	15	3
6	0	11.25	37.5	125	25	15	3
7	0	9	37.5	125	25	15	3
8	0	6.75	37.5	125	25	12	3
9	0	6.75	37.5	125	25	15	6
10	0	0	0	0	0	0	0

^*The Vickers salt solution contains 0.05 g/L FeSO₄, 0.05 g/LMnSO₄, 5.0 g/L K₂HPO₄, 2.5 g/L MgSO₄ and 2.5 g/L NaCl
^*Trace elements contains 5.5 g/L CaCl₂, 5.06 g/L MnCl₂, 1.1 g/L (NH₄)₂MoO₄, 1.57 g/L CuSO₄, 50.0 g/L EDTA, 2.2 g/L ZnSO4, 5.0 g/L FeSO₄ and 1.61 g/L CoCl₂