Table 2 The codon optimized nucleotide sequence for the synthesis and cloned in PET28a vector with restriction sites
From: Efficient strategy to isolate exosomes using anti-CD63 antibodies conjugated to gold nanoparticles
Name | Nucleotide sequence with restriction enzyme site | Accession no |
|---|---|---|
CD9 | Nco1:CCATGGGCTGGCTGCGTTTCGACTCTCAGACCAAATCTATCTTCGAGCAAGAAACCAACAACAACAATAGCTCCGGCGGTGGTGGCTCTTATTCTCATAAAGACGAAGTTATTAAAGAAGTGCAGGAATTTTACAAAGACACCTATAACAAACTGAAAACCAAAGACGAGCCGCAGCGCGAAACCCTGAAAGCAATTCACTACGCACTGAACTGTTGCGGCCTGGCAGGTGGTGTGGAGCAGTTCATTTCTGACATCTGCCCGAAAAAAGACGTTCTGGAAACTTTTACCGTTAAGAGCTGCCCGGACGCGATCAAAGAGGTGTTCGATAACAAATTCCACATTCTCGAG:Xho-1 | OR166431 |
CD63 | Nco1:CCATGGGCGTTGGTGCACAACTGGTTCTGTCTCAAACCATCATCCAGGGTGCTACCCCAGGTTCCGGTGGTGGTGGTAGCGGTTACGTATTCCGCGACAAAGTCATGTCCGAGTTCAATAACAACTTCCGTCAGCAGATGGAAAACTACCCGAAAAACAACCACACCGCAAGCATTCTGGACCGCATGCAGGCTGACTTCAAATGCTGCGGCGCAGCGAACTACACCGATTGGGAGAAAATTCCGTCCATGAGCAAAAACCGTGTACCGGACAGCTGTTGTATCAACGTTACTGTAGGTTGCGGCATCAACTTTAACGAAAAAGCTATCCACAAAGAAGGTTGCGTGGAAAAAATCGGTGGTTGGCTGCGTAAAAACGTGCTCGAG:Xho-1 | OR166432 |
CD81 | Nco1:CCATGGGCTGGCTGCGTCACGATCCTCAGACTACTAACCTGCTGTATCTGGAACTGGGTGATAAACCGGCGCCGAACACCTTTTACGTTGGCATTTACATCGGCGGTGGTGGCTCCGGTTTCGTTAACAAAGACCAGATTGCAAAAGATGTGAAACAGTTCTACGACCAGGCCCTGCAGCAGGCGGTTGTTGACGATGACGCGAACAACGCCAAGGCGGTTGTTAAGACTTTCCACGAGACCCTGGATTGCTGTGGTTCTAGCACCCTGACTGCGCTGACTACCTCTGTTCTGAAAAACAACCTGTGTCCATCTGGTTCTAACATTATCTCTAACCTGTTCAAAGAAGATTGCCACCAGAAAATCGATGACCTGTTTTCCGGTAAACTGCTCGAG:Xho-1 | OR166433 |