Fig. 3
From: Investigating the initial steps of auricin biosynthesis using synthetic biology
a Schemes of the integration plasmids pKasOp-aur2ABtFCGHA, pKasOp-aur2ABtlanCFDLE, pKasOp-aur1DEtFCGHA, and pKasOp-aur1DEtlanCFDLE, containing mixed auricin/landomycin artificial operons. Sequences upstream of individual genes are shown with RBS from pMU1s* (Craney et al. 2007) in green and the ATG codon underlined. Description of the plasmid backbone is in the legend to Fig. 1. Relevant restriction sites are shown. b HPLC analysis of metabolites produced by representative clones from the indicated S. coelicolor M1146 strains containing integrated plasmids after growth in liquid Bennet medium for 3 days. The rabelomycin peak is indicated by an arrow