Fig. 1
From: Investigating the initial steps of auricin biosynthesis using synthetic biology
a Pathway of the initial steps in the biosynthesis of landomycin to form the shunt product rabelomycin (Kharel et al. 2012a). b Schemes of the integration plasmids pErmEp-lanABCFDLE and pKasOp-lanABCFDLE, which contain an artificial lanABCFDL operon under the control of the ermEp* or kasOp* promoter. Wild-type lanEFABCDL central region encoding oxygenase (OXY), angucycline-specific cyclase (CYC), ketosynthase α (KSα), ketosynthase β (KSβ), acyl carrier protein (ACP), ketoreductase (KR), and aromatase (ARO), is shown above the diagrams. Sequences upstream of individual genes are shown with the ribosome binding site (RBS) from pMU1s* (Craney et al. 2007) in green and the ATG codon underlined. The plasmid backbone contains the fdT terminator (dark grey box), the E. coli origin of replication ColE1 (light grey box), the AprR aac3(IV) gene (blue arrow), the oriT origin of transfer (red bar), and the phiBT1int integrase gene (green arrow) together with the attP site (green bar) from plasmid pMU1s*. Relevant restriction sites are shown. c HPLC analysis of metabolites produced by representative clones from the indicated S. coelicolor M1146 strains containing integrated plasmids after growth in liquid Bennet medium for 3 days. The rabelomycin peak is indicated by an arrow