Table 3 Degradation behaviour of different deletion strains. Shown are the strain name, the deleted gene name, and the protein name of the corresponding protein. Strains were cultured in 10 mL LPU media supplemented with 125 mg/L cinnamic acid in 100 mL shake flasks at 30 °C and 220 rpm for 22 h. Cinnamic and p-coumaric acid show the average concentration in mg/L ± standard deviation of duplicates. 4-HBA was not detected in any of the samples
From: Cinnamic acid and p-coumaric acid are metabolized to 4-hydroxybenzoic acid by Yarrowia lipolytica
strain | deletion | protein name | OD600 | cinnamic acid [mg/L] | p-coumaric acid [mg/L] |
|---|---|---|---|---|---|
OKYL029 | none | 13.7 ± 0.4 | 0.0 ± 0.0 | 24.2 ± 0.3 | |
ICYL06 | YALI1_A15544g | Alk7 | 8.9 ± 0.6 | 9.1 ± 0.4 | 14.1 ± 0.6 |
ICYL07 | YALI1_F02132g | uncharacterized Protein | 9.1 ± 0.8 | 11.5 ± 2.9 | 13.7 ± 2.3 |
ICYL08 | YALI1_F05415g | Alk2 | 8.4 ± 0.1 | 13.0 ± 0.0 | 12.3 ± 1.0 |
ICYL09 | YALI1_B28430g | Cytochrome P450 | 8.4 ± 0.0 | 13.7 ± 0.5 | 0.0 ± 0.0 |
ICYL10 | YALI1_A21175g | Cytochrome P450 | 10.2 ± 0.1 | 5.7 ± 0.7 | 16.0 ± 0.7 |
ICYL11 | YALI1_B18347g | Alk5 | 8.8 ± 0.0 | 10.7 ± 1.6 | 13.4 ± 0.9 |
ICYL12 | YALI1_E27649g | Alk3 | 10.5 ± 0.2 | 2.4 ± 2.4 | 22.6 ± 0.2 |
ICYL13 | YALI1_E17508g | uncharacterized Protein | 10.1 ± 0.1 | 6.2 ± 0.0 | 16.4 ± 0.5 |
ICYL14 | YALI1_B27057g | Cytochrome P450 | 9.5 ± 0.8 | 7.7 ± 2.8 | 16.6 ± 1.9 |
ICYL16 | YALI1_B02833g | Alk6 | 10.2 ± 0.7 | 6.5 ± 0.9 | 14.1 ± 0.6 |
ICYL17 | YALI1_A18344g | Cytochrome P450 | 8.4 ± 0.3 | 1.9 ± 0.6 | 15.3 ± 1.7 |
ICYL18 | YALI1_E30815g | Cytochrome P450 | 8.4 ± 0.4 | 10.7 ± 1.1 | 12.8 ± 0.2 |
ICYL19 | YALI1_C17031g | uncharacterized Protein | 8.1 ± 0.6 | 13.8 ± 2.1 | 8.2 ± 1.3 |
ICYL20 | YALI1_B18311g | uncharacterized Protein | 8.3 ± 0.7 | 14.9 ± 1.5 | 11.5 ± 0.7 |
ICYL21 | YALI1_B08321g | Cytochrome P450 | 10.1 ± 0.6 | 8.5 ± 1.2 | 12.1 ± 0.1 |