Fig. 3
From: Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system
PCR-lbCas12a can be used to identify the drug resistant mutation. (a) Schematic of PCR primer design for the mutant site lack of PAM sequence. (b) Schematic of mutant regions and crRNA sequences used for detection. Mutant site in the target are highlighted as orange; crRNA for mutant sequence detection is colored as red. (c, d) Specificity detection of parC S80I crRNA. (n = 3 technical replicates; ***, p < 0.001; bars represent mean ± s.e.m.) (e) PCR-lbCas12a can detect Salmonella parC S80I at concentrations of 1 copy in 30 min (n = 3 technical replicates; ****, p < 0.0001; bars represent mean ± s.e.m.). (f) The electropherogram of the PCR product with gradient dilution plasmid template (copies)