Fig. 2
From: Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system
Detection efficacy of crRNAs with different preparation methods and modification. (a), (b) Comparation of detection curve between crRNAs (pagC1: left and invA1: right) with and without 5’ -phosphate. (c) Comparation of detection curve between pagC2-crRNAs obtained by chemical synthesis and in-vitro transcription. (n = 3 technical replicates; bars represent mean ± s.e.m.). (d) Fluorescence signal of chemically synthesized pagC2-crRNA is significantly higher than that in-vitro transcribed at 60 min (n = 3 technical replicates; **, p < 0.01, bars represent mean ± s.e.m.). (e) Comparation of detection curve between invA1-crRNAs obtained by chemical synthesis and in-vitro transcription. (n = 3 technical replicates; bars represent mean ± s.e.m.), (f) Fluorescence signal of chemically synthesized invA1-crRNA is significantly higher than that in-vitro transcribed at 60 min (n = 3 technical replicates; ***, p < 0.001, bars represent mean ± s.e.m.)