Fig. 1
From: Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system
Establishment of PCR-lbCas12a assay for Salmonella DNA detection. (a) Schematic of PCR-lbCas12a. (b) Positive ranking of G−bacilli of Shenzhen Luohu Hospital Group in 2021(left) and 2020(right). (c) Screening of crRNAs for Salmonella DNA detection by lbCas12a collateral detection. Represent one of three independent experiments. (d) PCR-lbCas12a can detect Salmonella at concentrations of 1 copy in 30 min through the lbCas12a cleavage reaction (n = 3 technical replicates; *, p < 0.05, **, p < 0.01, ****, p < 0.0001; bars represent mean ± s.e.m.). (e) Agarose gel electrophoresis for the PCR products of different Salmonella standard DNA (copies)