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Table 3 The OD yield response values obtained for the Yleh catalyzed reaction from two-level factorial experiments

From: Potential of Y. lipolytica epoxide hydrolase for efficient production of enantiopure (R)-1,2-octanediol

Run

A

B

C

D

Response

 

Substrate

Enzyme

Temperature

Reaction Time

OD

 

(mM)

(µg)

(°C)

(min)

(mM)

1

25

2

25

120

0.026

2

25

100

45

120

2.258

3

2.5

2

25

10

0.017

4

25

2

45

10

0.047

5

25

100

25

10

2.474

6

2.5

100

25

120

0.218

7

2.5

2

45

120

0.024

8

2.5

100

45

10

0.454

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