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AMB Express

Table 2 Predicted ORFs identified in phage vB_PaeP_PS28 phage

From: Isolation of a bacteriophage targeting Pseudomonas aeruginosa and exhibits a promising in vivo efficacy

Coding sequence

Start…..End

GC (%)

Protein length

MW (KDa)

Gene name

Putative function

Amino acid sequence identity/similarity to best homologs

BLAST score (E-Value)

Accession No.

ORF1

51…9608

56.25

3185

347.48

RNA polymerase

Transcription process and DNA replication

Virion-associated RNA polymerase [Pseudomonas phage PAP02]

0

QKE55114.1

ORF2

9624....9827

50.00

67

7.42

Hypothetical protein

Unknown function

Hypothetical protein FG40_gp65 [Pseudomonas phage vB_PaeP_C2-10_Ab09]

2e-07

YP_009031842.1

ORF3

9837…10154

57.55

105

11.47

Hypothetical protein

Unknown function

Hypothetical protein PP-LIT1_gp69 [Pseudomonas phage LIT1]

3e-70

YP_003358466.1

ORF4

10,15….10,387

55.27

78

8.88

Hypothetical protein

Unknown function

Hypothetical protein PAP02_040 [Pseudomonas phage PAP02]

1e-48

QKE55111.1

ORF5

10,687…10842

47.44

51

5.76

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage LP14]

3e-27

AWY02728.1

ORF6

10,793….11197

57.04

134

14.52

Hypothetical protein

Unknown function

Hypothetical protein ACQ34_gp70 [Pseudomonas phage YH6]

7e-94

YP_009152570.1

ORF7

11,255…11692

62.79

145

14.64

Hypothetical protein

Unknown function

Hypothetical protein vB_Pae575P-3_65 [Pseudomonas phage vB_Pae575P-3]

1e-39

ANT44344.1

ORF8

11,704…12264

54.19

186

20.41

N4 gp46-like protein

Structural protein (tubular tail A homologue)

N4 gp46-like protein [Pseudomonas phage LIT1]

1e-126

YP_003358462.1

ORF9

12,252….12695

56.31

147

17.15

N4 gp48-like protein

Structural protein (major capsid)

N4 gp48-like protein [Pseudomonas phage LIT1]

1e-87

YP_003358461.1

ORF10

12,692…13057

55.19

121

13.83

Hypothetical protein

Unknown function

Hypothetical protein BIZ95_gp65 [Pseudomonas phage vB_PaeP_MAG4]

2e-83

YP_009290599.1

ORF11

13,061…13807

58.90

248

26.78

Putative single-stranded DNA-binding protein

DNA replication

Putative single-stranded DNA-binding protein [Pseudomonas aeruginosa]

1e-157

SBT96838.2

ORF12

13,834…14568

58.90

244

28.08

Hypothetical protein

Unknown function

Hypothetical protein FDH24_gp59 [Pseudomonas phage PA26]

0.0

YP_009598413.1

ORF13

14,617…16785

58.90

722

82.15

Hypothetical protein

Unknown function

Hypothetical protein BIZ95_gp62 [Pseudomonas phage vB_PaeP_MAG4]

0.0

YP_009290596.1

ORF14

16,800…17810

54.60

336

37.8

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0077 [Pseudomonas phage vB_PaeP_FBPa1]

0.0

UVN14432.1

ORF15

17,812…18192

56.69

126

14.4

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage vB_PaeS_TUMS_P81]

6e-81

UGL60992.1

ORF16

18,189…18617

57.58

142

15.76

Hypothetical protein

Unknown function

Hypothetical protein ACQ34_gp60 [Pseudomonas phage YH6]

4e-99

YP_009152560.1

ORF17

18,820…18990

53.80

56

6.38

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0074 [Pseudomonas phage vB_PaeP_FBPa1]

1e-30

UVN14429.1

ORF18

19,011…20300

52.44

429

46.32

Tail fiber protein

Phage assembly (Tail morphogenesis)

Tail fiber protein [Pseudomonas phage vB_Pae575P-3]

0.0

ANT44334.1

ORF19

20,297…20596

50.67

99

11.7

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0072 [Pseudomonas phage vB_PaeP_FBPa1]

4e-65

UVN14427.1

ORF20

20,593…21264

50.45

223

24.96

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0071 [Pseudomonas phage vB_PaeP_FBPa1]

4e-161

UVN14426.1

ORF21

21,303…24560

55.03

1085

118.09

Tail fiber protein

Phage assembly (Tail morphogenesis)

Putative tail fiber protein [Pseudomonas phage PEV2]

0.0

YP_009286275.1

ORF22

24,488…24781

49.66

97

11.17

Hypothetical protein

Unknown function

Hypothetical protein BIZ95_gp54 [Pseudomonas phage vB_PaeP_MAG4]

1e-50

YP_009290588.1

ORF23

24,915…25109

55.38

64

7.52

Hypothetical protein

Unknown function

Hypothetical protein BIZ95_gp53 [Pseudomonas phage vB_PaeP_MAG4]

4e-33

YP_009290587.1

ORF24

25,106…25630

58.29

174

19.6

Major capsid protein

Phage structural assembly

(Head morphogenesis)

Major capsid protein [Pseudomonas phage vB_PaeP_FBPa1]

1e-123

UVN14423.1

ORF25

25,627…26139

56.14

170

18.68

Endopeptidase protein

Host cell lysis gene

Endopeptidase protein [Pseudomonas phage PAP02]

1e-103

QKE55097.1

ORF26

26,202…26759

52.51

185

21.36

Hypothetical protein

Unknown function

Hypothetical protein vBPaeSVL1_52 [Pseudomonas phage VB_PaeS_VL1]

1e-128

UGV19848.1

ORF27

26,752….26943

50.00

63

7.2

HNH endonuclease

Phage DNA packaging

HNH endonuclease [Pseudomonas phage YH30]

1e-32

YP_009226156.1

ORF28

26,993…27310

51.57

105

11.84

Hypothetical protein

Unknown function

Hypothetical protein PP-LIT1_gp45 [Pseudomonas phage LIT1]

3e-69

YP_003358442.1

ORF29

27,394…27618

50.67

74

8.86

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0063 [Pseudomonas phage vB_PaeP_FBPa1]

2e-35

UVN14418.1

ORF30

27,666….29435

60.17

589

63.4

Putative rIIB-like protein

Lysis inhibition, interfere with cell metabolism

Putative rIIB-like protein [Pseudomonas phage VB_PaeS_VL1]

0.0

UGV19844.1

ORF31

29,447…31975

55.36

842

95.11

Putative rIIA-like protein

Lysis inhibition, interfere with cell metabolism

Putative rIIA-like protein [Pseudomonas phage YH6]

0.0

YP_009152547.1

ORF32

31,979…32170

56.77

63

7.2

HNH endonuclease

Phage DNA packaging

HNH endonuclease [Pseudomonas phage YH6]

5e-40

YP_009152546.1

ORF33

32,167…32565

55.39

132

14.55

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0059 [Pseudomonas phage vB_PaeP_FBPa1]

5e-78

UVN14414.1

ORF34

32,740…33309

55.61

189

20.62

Putative dCMP deaminase

Nucleotide metabolism and DNA replication

Putative dCMP deaminase [Pseudomonas phage VB_PaeS_VL1]

2e-113

UGV19839.1

ORF35

33,391…36006

54.51

871

97.96

Putative DNA polymerase

DNA replication

Putative DNA polymerase [Pseudomonas phage LP14]

0.0

AWY02758.1

ORF36

36,006…36533

54.92

175

19.98

Hypothetical protein

Unknown function

Hypothetical protein PAP02_016 [Pseudomonas phage PAP02]

2e-125

QKE55087.1

ORF37

36,533…37699

56.64

388

44.08

Putative DNA helicase

DNA replication

Putative DNA helicase [Pseudomonas phage Pa2]

0.0

YP_009148216.2

ORF38

37,789…39015

56.40

408

45.58

Putative metallopeptidase domain

Lysis bacterial cell wall peptidoglycan

Putative metallopeptidase domain [Pseudomonas phage YH6]

0.0

YP_009152539.1

ORF39

39,015…39527

46.59

170

18.9

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0052 [Pseudomonas phage vB_PaeP_FBPa1]

7e-121

UVN14407.1

ORF40

39,539…40609

57.80

356

40.04

ATPase

Energy production during phage DNA packaging

ATPase [Pseudomonas phage YH30]

0.0

YP_009226170.1

ORF41

40,641…40883

60.08

80

9.02

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0050 [Pseudomonas phage vB_PaeP_FBPa1]

6e-52

UVN14405.1

ORF42

40,880…41713

57.67

277

30.99

ATP-dependent Clp protease ATP-binding subunit

Energy dependent protein degradation

ATP-dependent Clp protease ATP-binding subunit [Pseudomonas phage VB_PaeS_VL1]

0.0

UGV19832.1

ORF43

41,772…41957

49.46

61

7.07

Hypothetical protein

Unknown function

Hypothetical protein FDH24_gp30 [Pseudomonas phage PA26]

4e-37

YP_009598384.1

ORF44

41,966…42196

44.16

76

8.4

Hypothetical protein

Unknown function

Hypothetical protein vB_pae575P-3_30a [Pseudomonas phage vB_Pae575P-3]

2e-31

ANT44308.1

ORF45

42,434…42730

55.56

98

11.44

Hypothetical protein

Unknown function

Hypothetical protein BIZ95_gp30 [Pseudomonas phage vB_PaeP_MAG4]

5e-45

YP_009290564.1

ORF46

42,652…43161

54.31

169

18.96

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage LY218]

3e-65

QHZ59466.1

ORF47

43,158…43739

53.44

193

21.97

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage LP14]

1e-90

AWY02768.1

ORF48

43,739…44293

54.41

184

21.71

Hypothetical protein

Unknown function

Hypothetical protein FDH24_gp26 [Pseudomonas phage PA26]

3e-129

YP_009598380.1

ORF49

44,290…44916

52.95

208

24.47

Hypothetical protein

Unknown function

Hypothetical protein BIZ95_gp26 [Pseudomonas phage vB_PaeP_MAG4]

3e-142

YP_009290560.1

ORF50

44,920…45144

58.67

74

8.18

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0041 [Pseudomonas phage vB_PaeP_FBPa1]

8e-19

UVN14396.1

ORF51

45,217…45369

49.67

50

5.7

Membrane protein

Lysis protein

Membrane protein [Pseudomonas phage PAP02]

2e-27

QKE55073.1

ORF52

45,467…46708

55.72

413

47.13

RNA polymerase large subunit

Transcription and mRNA processing

Putative RNA polymerase II (RNAP2) [Pseudomonas phage Pa2]

0.0

ANT44300.1

ORF53

46,740…47015

42.39

91

10.73

Hypothetical protein

Unknown function

Hypothetical protein PAP02_080 [Pseudomonas phage PAP02]

3e-09

QKE55151.1

ORF54

47,020…47310

43.99

96

11.48

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0037 [Pseudomonas phage vB_PaeP_FBPa1]

1e-54

UVN14392.1

ORF55

47,315…47575

49.81

86

10.08

Hypothetical protein

Unknown function

Hypothetical protein vB_Pae575P-3_21 [Pseudomonas phage vB_Pae575P-3]

7e-53

ANT44297.1

ORF56

47,588…48520

50.16

310

35.94

RNA polymerase small subunit

Transcription of phage protein

RNA polymerase small subunit [Pseudomonas phage YH30]

0.0

YP_009226181.1

ORF57

48,532…48882

48.72

116

13.49

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0034 [Pseudomonas phage vB_PaeP_FBPa1]

7e-79

UVN14389.1

ORF58

48,915…49355

56.92

146

16.18

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0033 [Pseudomonas phage vB_PaeP_FBPa1]

2e-105

UVN14388.1

ORF59

49,355…49606

54.37

83

9.62

Transcriptional regulator

Transcriptional regulator

Transcriptional regulator [Pseudomonas phage YH30]

6e-50

YP_009226097.1

ORF60

49,606…50007

57.46

133

14.48

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage LP14]

1e-82

AWY02783.1

ORF61

50,007…50372

54.64

121

13.24

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0030 [Pseudomonas phage vB_PaeP_FBPa1]

2e-56

UVN14385.1

ORF62

50,557…50847

61.86

96

10.65

Hypothetical protein

Unknown function

Hypothetical protein FG40_gp12 [Pseudomonas phage vB_PaeP_C2-10_Ab09]

2e-60

YP_009031789.1

ORF63

50,939…51124

56.99

61

6.91

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0028 [Pseudomonas phage vB_PaeP_FBPa1]

1e-35

UVN14383.1

ORF64

51,197…51418

53.60

73

8.03

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage CMS1]

5e-41

UNY40717.1

ORF65

51,415…51648

56.84

77

8.57

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage phi176]

3e-44

AIZ94943.1

ORF66

51,645…51866

59.01

73

7.97

Hypothetical protein

Unknown function

Hypothetical protein PAP02_067 [Pseudomonas phage PAP02]

6e-31

QKE55138.1

ORF67

51,895…52098

56.37

67

7.3

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0024 [Pseudomonas phage vB_PaeP_FBPa1]

2e-28

UVN14379.1

ORF68

52,102…52398

54.21

98

11.26

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0023 [Pseudomonas phage vB_PaeP_FBPa1]

3e-63

UVN14378.1

ORF69

52,382…52690

50.81

102

12.08

Wall-associated receptor kinase-like 20-like protein

DNA metabolism and replication

Wall-associated receptor kinase-like 20-like protein [Pseudomonas phage YH30]

1e-65

YP_009226107.1

ORF70

52,687…52938

47.62

83

9.83

Hypothetical protein

Unknown function

Hypothetical protein P3P1_05 [Pseudomonas aeruginosa]

3e-35

SBT96754.2

ORF71

52,935…53168

49.15

77

8.95

Putative transcriptional regulator

Transcriptional regulator

Putative transcriptional regulator [Pseudomonas phage VB_PaeS_VL1]

2e-28

UGV19802.1

ORF72

53,165…53395

50.22

76

8.71

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0021 [Pseudomonas phage vB_PaeP_FBPa1]

3e-49

UVN14376.1

ORF73

53,410…53601

48.96

63

7.3

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0020 [Pseudomonas phage vB_PaeP_FBPa1]

2e-39

UVN14375.1

ORF74

54,016…54270

52.55

84

9.6

Hypothetical protein

Unknown function

Hypothetical protein FG40_gp01 [Pseudomonas phage vB_PaeP_C2-10_Ab09]

1e-50

YP_009031778.1

ORF75

54,691…55341

48.39

216

24.19

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage LP14]

5e-155

AWY02705.1

ORF76

55,543…55851

57.93

102

11.45

Hypothetical protein

Unknown function

Hypothetical protein FDH24_gp87 [Pseudomonas phage PA26]

3e-57

YP_009598441.1

ORF77

55,827….56102

53.62

91

10.68

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage vB_PaeP_TUMS_P121]

4e-34

UEP18636.1

ORF78

56,099….56311

50.23

70

7.73

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0014 [Pseudomonas phage vB_PaeP_FBPa1]

5e-38

UVN14369.1

ORF79

56,311….56742

53.70

143

16.1

Hypothetical protein

Unknown function

Hypothetical protein BIZ95_gp89 [Pseudomonas phage vB_PaeP_MAG4]

2e-93

YP_009290623.1

ORF80

56,809…57537

53.36

242

27.32

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0012 [Pseudomonas phage vB_PaeP_FBPa1]

2e-179

UVN14367.1

ORF81

57,534….59186

53.18

550

62.77

Terminase large subunit

Packing process and phage assembly

Terminase large subunit [Pseudomonas phage vB_PaeP_FBPa1]

0.0

UVN14366.1

ORF82

59,183…59917

50.88

244

27.91

Putative tail protein

Phage assembly (Tail morphogenesis)

Putative tail protein [Pseudomonas phage YH30]

8e-178

YP_009226120.1

ORF83

59,950….60249

56.00

99

11.1

ABC transporter-like protein

Mediate translocation to cell surface

ABC transporter-like protein [Pseudomonas phage YH30]

5e-63

YP_009226121.1

ORF84

60,253…60672

55.00

139

14.98

Putative dUTPase

DNA replication and metabolism

Putative dUTPase [Pseudomonas phage PEV2]

8e-94

YP_009286305.1

ORF85

60,707…62887

54.20

726

81.66

Portal protein

Translocation of phage DNA and packaging process (virion assembly)

Portal protein [Pseudomonas phage DL64]

0.0

YP_009206215.1

ORF86

62,957….63295

56.05

112

13.08

Hypothetical protein

Unknown function

Hypothetical protein PP-LIT1_gp79 [Pseudomonas phage LIT1]

4e-65

YP_003358476.1

ORF87

63,295….64476

52.28

393

44.13

Hypothetical protein

Unknown function

Hypothetical protein vBPaeSVL1_82 [Pseudomonas phage VB_PaeS_VL1]

0.0

UGV19878.1

ORF88

64,511…65710

59.75

399

44.06

Major capsid protein

Phage structural assembly (Head morphogenesis)

Major capsid protein [Pseudomonas phage VB_PaeS_VL1]

0.0

UGV19877.1

ORF89

65,767….66432

59.01

221

25.02

Hypothetical protein

Unknown function

Hypothetical protein vB_Pae575P-3_75 [Pseudomonas phage vB_Pae575P-3]

1e-123

ANT44354.1

ORF90

66,436…67401

55.49

321

35.21

Structural protein

Structural protein

Structural protein [Pseudomonas phage vB_Pae575P-3]

0.0

ANT44353.1

ORF91

67,459…69681

53.13

740

82.22

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage vB_PaeS_TUMS_P81]

0.0

UGL60976.1

ORF92

69,662…70129

57.26

155

16.64

Hypothetical protein

Unknown function

Hypothetical protein FBPa1_0091 [Pseudomonas phage vB_PaeP_FBPa1]

3e-95

UVN14446.1

ORF93

70,129…71694

55.43

521

57.27

Putative lytic tail fiber protein

Structural protein (Tail fiber morphogenesis)

Lytic tail fiber [Pseudomonas phage YH30]

0.0

YP_009226131.1

ORF94

71,674…72150

57.02

158

16.78

Hypothetical protein

Unknown function

Hypothetical protein [Pseudomonas phage LY218]

4e-65

QHZ59508.1

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