Table 2 Effect of deletion of phaABC genes from the SoF1 genome on CDW and PHB content of the cells. Cells were grown autotrophically in shake flasks in modified DSMZ81 media. Sampling was performed at the end of the cultivation when ammonia had been depleted for 3 to 4 days. Average from three shake flasks is shown with standard deviation
Strain | OD600 | CDW (g/L) | PHB (%CDW) |
|---|---|---|---|
SoF1 | 8.8 ± 0.4 | 4.7 ± 0.1 | 6.3 ± 0.9 |
SoF1ΔphaA | 7.8 ± 0.2 | 3.9 ± 0.1 | 2.8 ± 0.3 |
SoF1ΔphaB | 8.3 ± 0.3 | 4.6 ± 0.2 | 0.6 ± 0.1 |
SoF1ΔphaC | 7.8 ± 0.2 | 3.4 ± 0.1 | 0.0 ± 0.0 |
SoF1ΔphaC2 | 9.1 ± 0.4 | 2.6 ± 1.3 | 2.9 ± 0.7 |