Table 1 Plasmids and bacterial strains used in the study
Plasmid | Description | Reference/source |
|---|---|---|
pUC57 | E. coli cloning vector with an ampicillin marker | GenBank: Y14837.1 |
pSF00A | pUC57 with phaA::kan integration cassette | This study |
pSF00B | pUC57 with phaB::kan integration cassette | This study |
pSF00C | pUC57 with phaC1::kan integration cassette | This study |
pSF00D | pUC57 with phaC2::tet integration cassette | This study |
Strain | ||
Xanthobacter sp. SoF1 | Wild type | VTT E-193585 (Holmström and Pitkänen 2021) |
SoF1ΔphaA | SoF1 derivative; phaA::kan | This study |
SoF1ΔphaB | SoF1 derivative; phaB::kan | This study |
SoF1ΔphaC | SoF1 derivative; phaC1::kan | This study |
SoF1ΔphaC2 | SoF1 derivative; phaC2::tet | This study |