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Table 1 Comparison of the effects of different culture conditions/factors on growth and vitamin D3 bioconversion activity of Actinomyces hyovaginalis isolate A11-2, in a laboratory fermenter

From: Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2

Run #

Inoculum Size (% v/v)

Timing of substrate addition timing after main culture beginning (days)

Aeration rate (vvm)

Agitation rate (rpm)

pH control (a)

C source

N source

Mean Activity(c) ± S.D

Mean Growth (d) ± S.D

Specific activity (e)

Run 1

2

2

1

200

Uncontrolled

Fructose

Soybean

16.4 ± 0.22

6.9 ± 0.17

47.4

Run 2

2

2

0.1

200

Uncontrolled

Fructose

Soybean

3.4 ± 0.15

2.6 ± 0.15

26.0

Run 3

2

2

2

200

Uncontrolled

Fructose

Soybean

5.5 ± 0.13

7.8 ± 0.2

14.0

Run 4

2

2

1

400

Uncontrolled

Fructose

Soybean

15.5 ± 0.22

8.6 ± 0.35

36.0

Run 5

4

2

1

200

Uncontrolled

Fructose

Soybean

12.3 ± 0.31

9.1 ± 0.17

27.0

Run 6

2

2

1

200

Uncontrolled

Fructose

Skim milk

9.2 ± 0.22

5.5 ± 0.12

33.4

Run 7

2

2

1

200

Uncontrolled

Glucose

Soybean

10.8 ± 0.15

5.7 ± 0.15

3.00

Run 8

2

3

1

200

Uncontrolled

Fructose

Soybean

8.9 ± 0.16

9.9 ± 0.32

18.0

Run 9

2

2

1

200

Controlled at 7.8 (b)

Fructose

Soybean

4.2 ± 0.32

4.5 ± 0.12

18.0

  1.  S.D. (standard deviation)
  2. aInitial pH was 7.8
  3. b pH control was done at the same time of substrate addition
  4. c Expressed in terms of the amount of calcitriol produced (µg/50 mL)
  5. dExpressed in terms of viable count (X 106 CFU/mL)
  6. eExpressed in terms of the amount of calcitriol produced (ng) per 1 million CFU
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