Table 1 Bacterial strains and plasmids used or developed in this study
Strain | Description | Reference |
|---|---|---|
E. coli TOP10 | Fˊ(lacIq Tn10 (tetR)) mcrAD ΔlacX74 deoR nupG recA1 araD139Δ(ara-leu)7697 galU galK rpsL (strR) endA1λ- propagation host | Invitrogen |
B. subtilis 168 | trpC2 (ATCC 33,712), (ATCC, Manassas, VA, USA) | (Harwood 1992) |
RBSHA | B. subtilis 168 carrying pDHhas | This study |
RBSHA2 | B. subtilis168 carrying pDHhas and pG2 | This study |
RBSHA3 | B. subtilis 168 carrying pDHhas and pG3 | This study |
Plasmids | Description | Reference |
pDG148 | Multicopy B. subtilis and E. coli shuttle vector; Apr, Kmr, replicative | (Stragier et al. 1988) |
pG1 | pDG148 carrying hasA gene | This study |
pG2 | pDG148 carrying tuaD and gtaB genes | This study |
pG3 | pDG148 carrying tuaD, gtaB, and vgb genes | This study |
pDHAFB | B. subtilis and E. coli shuttle vector; Apr, amy E:[ Cmr, lacZ, Pspac] | (Yamamoto et al. 1999) |
pDHhas | pDHAFB carrying hasA gene | This study |