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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: The effect of manipulating glucuronic acid biosynthetic pathway in Bacillus subtilis strain on hyaluronic acid production

Fig. 2

Phenotypic and genotypic study of pDHhas integration in the genome of B. subtilis. (a) Amylase function study in the recombinant B. subtilis. Wells 1, 3, 4, and 6 are recombinant clone harboring pDHhas and wells 2 and 5 are LB broth medium minus bacteria before (left) and after (right) pouring iodine solution. (b) Amylase function study in recombinant B. subtilis. Lanes 1, 3, 4, and 6 are recombinant clones harboring pDHhas and lanes 2 and 5 are LB broth medium minus bacteria before (left) and after (right) pouring iodine solution. (c) Agarose gel electrophoresis of hasA PCR product from RBSHA genome [Lanes 1 to 4: PCR product of four RBSHA clones with hasA-F and hasA-R primers, confirmed by phenotypic test; lane 5: PCR product of RBSHA clone with hasA-F, hasA-IF, and hasA-R primers, confirmed by phenotypic test; M: 1 kb DNA size marker]. PCR amplified hasA genes with the expected length of 1500 bp are shown in lanes 1, 2, 3, and 4

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