Table 4 Purification of C-K from fermentation broth via ethanol and resin treatments
Step | Volume (L) | Dried solid (g) | C-K (g) | C-K concentration (g/L) | Purity (%) | Yield (%) | Recovery (%) |
|---|---|---|---|---|---|---|---|
Fermentation broth | 1.00 | 1.92 ± 0.00 | 100 | ||||
1st Ethanol extraction | 1.00 | 2.35 ± 0.02 | 0.87 ± 0.01 | 0.87 ± 0.01 | 37.0 ± 0.11 | 45.3 ± 0.52 | 45.3 ± 0.52 |
2nd Ethanol extraction | 1.00 | 0.98 ± 0.01 | 0.34 ± 0.01 | 0.34 ± 0.01 | 34.7 ± 0.16 | 32.4 ± 0.95 | 63.0 ± 1.76 |
3rd Ethanol extraction | 1.00 | 0.60 ± 0.01 | 0.13 ± 0.02 | 0.13 ± 0.01 | 21.7 ± 1.23 | 18.3 ± 2.82 | 69.8 ± 3.12 |
Supernatant extraction | 1.00 | 0.31 ± 0.02 | 0.08 ± 0.01 | 0.08 ± 0.01 | 25.8 ± 0.05 | 4.17 ± 0.52 | 74.0 ± 1.74 |
All extraction | 0.20 | 4.24 ± 0.05 | 1.42 ± 0.01 | 7.10 ± 0.05 | 33.5 ± 0.65 | 74.0 ± 0.52 | 74.0 ± 0.52 |
Ultrafiltration | 0.05 | 1.85 ± 0.03 | 1.09 ± 0.01 | 218 ± 6.00 | 58.9 ± 0.43 | 76.8 ± 0.70 | 56.8 ± 0.56 |
Octadecyl-silica resin | 0.03 | 1.21 ± 0.03 | 0.85 ± 0.01 | 28.3 ± 0.33 | 70.2 ± 1.08 | 77.9 ± 0.92 | 44.3 ± 0.64 |
C18 resin in prep-HPLC | 0.01 | 0.50 ± 0.01 | 0.48 ± 0.01 | 48.0 ± 1.10 | 96.0 ± 0.04 | 56.4 ± 1.18 | 25.0 ± 0.59 |