Production of ginsenoside compound K from American ginseng extract by fed-batch culture of Aspergillus tubingensis

AMB Express

Table 1 Effect of initial sucrose concentration on compound K (C-K) production in batch fermentation of Aspergillus tubingensis

Initial sucrose (g/L)	X_max (g/L)	μ_max (1/h)	Activity^a (U/mL)	C-K_max (g/L)	Y_P/S (g/g)	Y_P/AGE (g/g)	q_P (mg/L/h)	Molar conversion (%)	f_t (h)
10	18.6 ± 0.2	0.31 ± 0.003	5.64 ± 0.05	0.99 ± 0.01	0.09 ± 0.001	0.06 ± 0.001	6.88 ± 0.06	25.7 ± 0.26	144
20	19.8 ± 0.3	0.33 ± 0.006	6.43 ± 0.04	1.29 ± 0.02	0.07 ± 0.001	0.08 ± 0.001	8.96 ± 0.10	33.6 ± 0.39	144
30	23.7 ± 0.4	0.33 ± 0.006	3.65 ± 0.04	0.83 ± 0.02	0.03 ± 0.001	0.05 ± 0.001	5.32 ± 0.13	21.6 ± 0.52	156
40	31.2 ± 0.5	0.52 ± 0.004	1.76 ± 0.05	0.71 ± 0.02	0.02 ± 0.001	0.04 ± 0.001	4.55 ± 0.09	18.5 ± 0.39	156
50	26.4 ± 0.3	0.44 ± 0.006	0.94 ± 0.04	0.29 ± 0.02	0.01 ± 0.001	0.02 ± 0.001	1.86 ± 0.02	7.55 ± 0.65	156

μ_max: maximum specific growth rate, Y_P/S: C-K yield from sucrose, Y_P/AGE: C-K yield from AGE, q_P: volumetric production rate of C-K, f_t: fermentation time
^aβ-Glucosidase activity was measured by the release of para-nitrophenol (pNP) from para-nitrophenyl β-d-glucopyranoside (pNPGlc)