Table 1 Primers for construction of plasmids
Name | Direction | Sequence(5’–3’) |
|---|---|---|
pCold-F2-F | Forward | CCGCTCGAGATGAAACGGACCAACAGATC |
pCold-F2-R | Reverse | CCGGAATTCCTAATTAACATTTGATGGGTTG |
pc-F2-F | Forward | AGCTTGGTACCGAGCATGAAACGGACCAACAGATC |
pc-F2-R | Reverse | ATATCTGCAGAATTCCTAATTAACATTTGATGGGTTG |
pc-F2-1-66aa-R | Reverse | ATATCTGCAGAATTCTTACGGGTCGGATGTTCTTACGT |
pc-F2-1-99aa-R | Reverse | ATATCTGCAGAATTCTTATAGAGGCCCGTTTGGGTCGA |
pc-F2-1-132aa-R | Reverse | ATATCTGCAGAATTCTTAGATAGGGCCACGTGGATCTG |
pc-F2-1-165aa-R | Reverse | ATATCTGCAGAATTCTTATAGTCGTACACCGAGAGTCA |
pc-F2-1-198aa-R | Reverse | ATATCTGCAGAATTCTTAACTAACGAGTACTGTTAGGC |
pc-F2-1-231aa-R | Reverse | ATATCTGCAGAATTCTTATAACTCTTTCTCTCCTTGAG |
pc-F2-1-264aa-R | Reverse | ATATCTGCAGAATTCTTATAGGGTATTGTCAGTGACGG |
pc-F2-1-297aa-R | Reverse | ATATCTGCAGAATTCTTACGTAACAGACCCTGCTCCGC |
pc-F2-1-330aa-R | Reverse | ATATCTGCAGAATTCTTATACAGCATGTGATGCATCCT |
pc-F2-1-363aa-R | Reverse | ATATCTGCAGAATTCTTAGTTGTTGGTACAGTGGGAAA |
pc-F2-1-396aa-R | Reverse | ATATCTGCAGAATTCTTACAGAATAAAGTCATGTTCTG |
pc-F2-1-429aa-R | Reverse | ATATCTGCAGAATTCTTATACTTGGGCTATGGAGAATC |
pc-F2-1-462aa-R | Reverse | ATATCTGCAGAATTCTTAATCACCAGTTGTGCCTTGTT |
pc-linear-F | Forward | GAATTCTGCAGATATCCAGCACAGTG |
pc-linear-R | Reverse | GCTCGGTACCAAGCTTAAGTTTAAACG |