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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Identification of novel B cell epitopes in Fiber-2 protein of duck adenovirus 3 and their application

Fig. 1

The expression of the recombinant Fiber-2 and its immunogenicity. The expressed and purified recombinant Fiber-2 was identified by SDS-PAGE (A) and Western blot (B) with mAb against His-Tag as primary antibody. M, PageRuler™ Prestained Protein Ladder; Lane 1: bacterial lysate of E. coli BL21 transfected with pCold-1; Lane 2: bacterial lysate of E. coli BL21 transfected with pCold-1-Fiber-2; Lane 3: His-Fiber-2 fusion protein purified from bacterial lysate of E. coli BL21 transfected with pCold-1-Fiber-2. The polyclonal antibody was identified with transfected or infected LMH cells by IFA (C) and Western blot (D). Lane 1: LMH cells transfected with pcDNA3.1; Lane 2: LMH cells transfected with pcDNA3.1-Fiber-2; Lane 3: LMH cells mock infected; Lane 4: LMH cells infected with DAdV-3.

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