Strain | Relevant genotype or properties | Source |
---|---|---|
P. thermoglucosidasius DSM2542/BGSC ID 95A1 | Wildtype isolate | Bacillus Genetic Stock Center (USA) |
Pth::pMM7 | DSM2542::pMM7 KanR | This work |
Pth ∆spo0A | DSM2542 Δspo0A | This work |
E. coli DH5α™ | F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- | ThermoFisher Scientific |
E. coli DH5α-λpir | F- φ80lacZ∆M15 ∆(lacZYA-argF)U169 recA1 endA1 hsdR17(rk-, mk +) phoA supE44 thi-1 gyrA96 relA1 λ-pir lysogen of DH5α | Lab collection |
Plasmid | Description | Source |
---|---|---|
pMTL61110 | E. coli/P. thermoglucosidasius integrative shuttle vector, Thermosensitive replicon | Sheng et al. 2017 |
pIP7 | Vector carrying sfGFP | Pogrebnyakov et al. 2017 |
pGB-sfGFP | pMTL61110 carrying P7-sfGFP | This work |
pGB-sfGFP-best | pGB-sfGFP (F11V, T13P, N39D, A179A) | This work |
pMM7 | pGB-sfGFP-best carrying flanking regions of spo0A | This work |