Fig. 1
From: Overexpression of miR-32 in Chinese hamster ovary cells increases production of Fc-fusion protein
Expression of FC-fusion in CHO-K1 cells and clonal selection results. a Schematic view of the pTracer-CMV2-VEGF-trap construct. b Indicates the result of fluorescent imaging of pTracerCMV2-Aflibercept transfected cells after antibiotic selection of cell pool. c Indicates the derivative single clones after limiting dilution by their Fc-fusion expression and population heterogeneity based on percentage of GFP positive population. More details of flow cytometry results are shown in Additional file 1: Fig. S1. Error bars represent standard deviation of technical replicate (n = 3) of ELISA test