Table 3 Primers used for the quantification of the specific bacterial divisions’ expression by real-time PCR
Target species | Primer sequence (5 → 3) | Annealing temp (°C) | Product size (bp) | Reference |
|---|---|---|---|---|
Total bacteria | F: CGGYCCAGACTCCTACGGG R: TTACCGCGGCTGCTGGCAC | 60.0 | 130 | Guo et al. (2008) |
Firmicutes | F: GGAGYATGTGGTTTAATTCGAAGCA R: AGCTGACGACAACCATGCAC | 64.5 | 126 | |
Bacteroidetes | F: GGARCATGTGGTTTAATTCGATGAT R: AGCTGACGACAACCATGCAG | 60.0 | 126 | |
Lactobacillus spp. | F: AGCAGTAGGGAATCTTCCA R: CACCGCTACACATGGAG | 64.5 | 341 | Walter et al. (2001) |
Enterococcus spp. | F: CCCTTATTGTTAGTTGCCATCATT R: ACTCGTTGTACTTCCCATTGT | 59.6 | 144 | Rinttilä et al. (2004) |
Streptococcus spp. | F: AGAGTTTGATCCTGGCTCAG R: GTTAGCCGTCCCTTTCTGG | 56.8 | 485 | Franks et al. (1998) |
Clostridium cluster I | F: ATGCAAGTCGAGCGAKG R: TATGCGGTATTAATCTYCCTTT | 60.0 | 120 | Rinttilä et al. (2004) |
Clostridium cluster IV | F: GCACAAGCAGTGGAGT R: CTTCCTCCGTTTTGTCAA | 60.0 | 230 | Matsuki et al. (2004) |
Clostridium cluster XIVa | F: AAATGACGGTACCTGACTAA R: CTTTGAGTTTCATTCTTGCGAA | 60.0 | 440 | Matsuki et al. (2002) |
Bifidobacterium | F: TCGCGTCYGGTGTGAAAG R: CCACATCCAGCRTCCAC | 56.9 | 243 | Rinttilä et al. (2004) |
Enterobacteriaceae | F: CATTGACGTTACCCGCAGAAGAAGC R: CTCTACGAGACTCAAGCTTGC | 62.5 | 195 | Bartosch et al. (2004) |