Fig. 2From: Biotransformation of ferulic acid to protocatechuic acid by Corynebacterium glutamicum ATCC 21420 engineered to express vanillate O-demethylaseTransformation assays of FA and VAN. C. glutamicum strain F was cultured in BHI medium at 30 °C for 24 h, and 0.1 mL of each culture was transferred to 5 mL of AR medium containing 2 mM each of FA or VAN with 25 μg/mL of Km in 5 mL tubes. The biotransformation of a FA and b VAN was performed at 30 °C with agitation at 180 rpm for 72 h. The concentrations of extracellular FA (squares), VA (triangles), VAN (diamonds) and PCA (circles) were monitored every 24 h. Data represent the mean and standard error from three independent experimentsBack to article page