Table 3 Immobilization of d-Hyd(co) and d-Carb via functionalized magnetic beads and hydrolysis of different substrates
Fraction | Substrate | d-Hyd(co) | Substrate | d-Carb | ||
|---|---|---|---|---|---|---|
cprot (mg/mL) | Aspez (mU/mg) | cprot (mg/mL) | Aspez (U/mg) | |||
Crude extract | PheHyd | 5.00 | 0.36 | NCPheGly | 5.00 | 0.07 |
Immobilized enzyme | 0.11 | 70.75 | 0.03 | 11.75 | ||
Crude extract | BnH | 5.00 | 0.28 | NCPheAla | 5.00 | 0.10 |
Immobilized enzyme | 0.08 | 63.20 | 0.03 | 12.91 | ||
Crude extract | HMH | 5.00 | 0.76 | NCSer | 5.00 | 0.08 |
Immobilized enzyme | 0.08 | 105.47 | 0.03 | 11.75 | ||